Cargando…

An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus

The remarkably adaptive mite Varroa destructor is the most important honey bee ectoparasite. Varroa mites are competent vectors of deformed wing virus (DWV), and the Varroa-virus complex is a major determinant of annual honey bee colony mortality and collapse. MicroRNAs (miRNAs) are 22-24 nucleotide...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar, Deepak, Alburaki, Mohamed, Tahir, Faizan, Goblirsch, Michael, Adamczyk, John, Karim, Shahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966896/
https://www.ncbi.nlm.nih.gov/pubmed/35372101
http://dx.doi.org/10.3389/fcimb.2022.847000
_version_ 1784678732177342464
author Kumar, Deepak
Alburaki, Mohamed
Tahir, Faizan
Goblirsch, Michael
Adamczyk, John
Karim, Shahid
author_facet Kumar, Deepak
Alburaki, Mohamed
Tahir, Faizan
Goblirsch, Michael
Adamczyk, John
Karim, Shahid
author_sort Kumar, Deepak
collection PubMed
description The remarkably adaptive mite Varroa destructor is the most important honey bee ectoparasite. Varroa mites are competent vectors of deformed wing virus (DWV), and the Varroa-virus complex is a major determinant of annual honey bee colony mortality and collapse. MicroRNAs (miRNAs) are 22-24 nucleotide non-coding RNAs produced by all plants and animals and some viruses that influence biological processes through post-transcriptional regulation of gene expression. Knowledge of miRNAs and their function in mite biology remains limited. Here we constructed small RNA libraries from male and female V. destructor using Illumina’s small RNA-Seq platform. A total of 101,913,208 and 91,904,732 small RNA reads (>18 nucleotides) from male and female mites were analyzed using the miRDeep2 algorithm. A conservative approach predicted 306 miRNAs, 18 of which were upregulated and 13 downregulated in female V. destructor compared with males. Quantitative real-time PCR validated the expression of selected differentially-expressed female Varroa miRNAs. This dataset provides a list of potential miRNA targets involved in regulating vital Varroa biological processes and paves the way for developing strategies to target Varroa and their viruses.
format Online
Article
Text
id pubmed-8966896
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89668962022-03-31 An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus Kumar, Deepak Alburaki, Mohamed Tahir, Faizan Goblirsch, Michael Adamczyk, John Karim, Shahid Front Cell Infect Microbiol Cellular and Infection Microbiology The remarkably adaptive mite Varroa destructor is the most important honey bee ectoparasite. Varroa mites are competent vectors of deformed wing virus (DWV), and the Varroa-virus complex is a major determinant of annual honey bee colony mortality and collapse. MicroRNAs (miRNAs) are 22-24 nucleotide non-coding RNAs produced by all plants and animals and some viruses that influence biological processes through post-transcriptional regulation of gene expression. Knowledge of miRNAs and their function in mite biology remains limited. Here we constructed small RNA libraries from male and female V. destructor using Illumina’s small RNA-Seq platform. A total of 101,913,208 and 91,904,732 small RNA reads (>18 nucleotides) from male and female mites were analyzed using the miRDeep2 algorithm. A conservative approach predicted 306 miRNAs, 18 of which were upregulated and 13 downregulated in female V. destructor compared with males. Quantitative real-time PCR validated the expression of selected differentially-expressed female Varroa miRNAs. This dataset provides a list of potential miRNA targets involved in regulating vital Varroa biological processes and paves the way for developing strategies to target Varroa and their viruses. Frontiers Media S.A. 2022-03-16 /pmc/articles/PMC8966896/ /pubmed/35372101 http://dx.doi.org/10.3389/fcimb.2022.847000 Text en Copyright © 2022 Kumar, Alburaki, Tahir, Goblirsch, Adamczyk and Karim https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Kumar, Deepak
Alburaki, Mohamed
Tahir, Faizan
Goblirsch, Michael
Adamczyk, John
Karim, Shahid
An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus
title An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus
title_full An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus
title_fullStr An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus
title_full_unstemmed An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus
title_short An Insight Into the microRNA Profile of the Ectoparasitic Mite Varroa destructor (Acari: Varroidae), the Primary Vector of Honey Bee Deformed Wing Virus
title_sort insight into the microrna profile of the ectoparasitic mite varroa destructor (acari: varroidae), the primary vector of honey bee deformed wing virus
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966896/
https://www.ncbi.nlm.nih.gov/pubmed/35372101
http://dx.doi.org/10.3389/fcimb.2022.847000
work_keys_str_mv AT kumardeepak aninsightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT alburakimohamed aninsightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT tahirfaizan aninsightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT goblirschmichael aninsightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT adamczykjohn aninsightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT karimshahid aninsightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT kumardeepak insightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT alburakimohamed insightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT tahirfaizan insightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT goblirschmichael insightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT adamczykjohn insightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus
AT karimshahid insightintothemicrornaprofileoftheectoparasiticmitevarroadestructoracarivarroidaetheprimaryvectorofhoneybeedeformedwingvirus