Cargando…
Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection
OBJECTIVE: To identify the feature of N6-methyladenosine (m6A) methylation modification genes in acute aortic dissection (AAD) and explore their relationships with immune infiltration. METHODS: The GSE52093 dataset including gene expression data from patients with AAD and healthy controls was downlo...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967178/ https://www.ncbi.nlm.nih.gov/pubmed/35369349 http://dx.doi.org/10.3389/fcvm.2022.831561 |
_version_ | 1784678785592852480 |
---|---|
author | Yin, Fanxing Zhang, Hao Guo, Panpan Wu, Yihao Zhao, Xinya Li, Fangjun Bian, Ce Chen, Chen Han, Yanshuo Liu, Kun |
author_facet | Yin, Fanxing Zhang, Hao Guo, Panpan Wu, Yihao Zhao, Xinya Li, Fangjun Bian, Ce Chen, Chen Han, Yanshuo Liu, Kun |
author_sort | Yin, Fanxing |
collection | PubMed |
description | OBJECTIVE: To identify the feature of N6-methyladenosine (m6A) methylation modification genes in acute aortic dissection (AAD) and explore their relationships with immune infiltration. METHODS: The GSE52093 dataset including gene expression data from patients with AAD and healthy controls was downloaded from Gene Expression Omnibus (GEO) database in order to obtain the differentially expressed genes (DEGs). The differentially methylated m6A genes were obtained from the GSE147027 dataset. The differentially expressed m6A-related genes were obtained based on the intersection results. Meanwhile, the protein-protein interaction (PPI) network of differentially expressed m6A-related genes was constructed, and hub genes with close relationships in the network were selected. Later, hub genes were verified by using the GSE153434 dataset. Thereafter, the relationships between these genes and immune cells infiltration were analyzed. RESULTS: A total of 279 differentially expressed m6A-related genes were identified in the GSE52093 and GSE147027 datasets. Among them, 94 genes were up-regulated in aortic dissection (AD), while the remaining 185 were down-regulated. As indicated by Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, these genes were mainly associated with extracellular matrix (ECM) and smooth muscle cells (SMCs). The seven hub genes, namely, DDX17, CTGF, FLNA, SPP1, MYH11, ITGA5 and CACNA1C, were all confirmed as the potential biomarkers for AD. According to immune infiltration analysis, it was found that hub genes were related to some immune cells. For instance, DDX17, FLNA and MYH11 were correlated with Macrophages M2. CONCLUSION: Our study identifies hub genes of AD that may serve as the potential biomarkers, illustrates of the molecular mechanism of AD, and provides support for subsequent research and treatment development. |
format | Online Article Text |
id | pubmed-8967178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89671782022-03-31 Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection Yin, Fanxing Zhang, Hao Guo, Panpan Wu, Yihao Zhao, Xinya Li, Fangjun Bian, Ce Chen, Chen Han, Yanshuo Liu, Kun Front Cardiovasc Med Cardiovascular Medicine OBJECTIVE: To identify the feature of N6-methyladenosine (m6A) methylation modification genes in acute aortic dissection (AAD) and explore their relationships with immune infiltration. METHODS: The GSE52093 dataset including gene expression data from patients with AAD and healthy controls was downloaded from Gene Expression Omnibus (GEO) database in order to obtain the differentially expressed genes (DEGs). The differentially methylated m6A genes were obtained from the GSE147027 dataset. The differentially expressed m6A-related genes were obtained based on the intersection results. Meanwhile, the protein-protein interaction (PPI) network of differentially expressed m6A-related genes was constructed, and hub genes with close relationships in the network were selected. Later, hub genes were verified by using the GSE153434 dataset. Thereafter, the relationships between these genes and immune cells infiltration were analyzed. RESULTS: A total of 279 differentially expressed m6A-related genes were identified in the GSE52093 and GSE147027 datasets. Among them, 94 genes were up-regulated in aortic dissection (AD), while the remaining 185 were down-regulated. As indicated by Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, these genes were mainly associated with extracellular matrix (ECM) and smooth muscle cells (SMCs). The seven hub genes, namely, DDX17, CTGF, FLNA, SPP1, MYH11, ITGA5 and CACNA1C, were all confirmed as the potential biomarkers for AD. According to immune infiltration analysis, it was found that hub genes were related to some immune cells. For instance, DDX17, FLNA and MYH11 were correlated with Macrophages M2. CONCLUSION: Our study identifies hub genes of AD that may serve as the potential biomarkers, illustrates of the molecular mechanism of AD, and provides support for subsequent research and treatment development. Frontiers Media S.A. 2022-03-14 /pmc/articles/PMC8967178/ /pubmed/35369349 http://dx.doi.org/10.3389/fcvm.2022.831561 Text en Copyright © 2022 Yin, Zhang, Guo, Wu, Zhao, Li, Bian, Chen, Han and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cardiovascular Medicine Yin, Fanxing Zhang, Hao Guo, Panpan Wu, Yihao Zhao, Xinya Li, Fangjun Bian, Ce Chen, Chen Han, Yanshuo Liu, Kun Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection |
title | Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection |
title_full | Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection |
title_fullStr | Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection |
title_full_unstemmed | Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection |
title_short | Comprehensive Analysis of Key m6A Modification Related Genes and Immune Infiltrates in Human Aortic Dissection |
title_sort | comprehensive analysis of key m6a modification related genes and immune infiltrates in human aortic dissection |
topic | Cardiovascular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967178/ https://www.ncbi.nlm.nih.gov/pubmed/35369349 http://dx.doi.org/10.3389/fcvm.2022.831561 |
work_keys_str_mv | AT yinfanxing comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT zhanghao comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT guopanpan comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT wuyihao comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT zhaoxinya comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT lifangjun comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT biance comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT chenchen comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT hanyanshuo comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection AT liukun comprehensiveanalysisofkeym6amodificationrelatedgenesandimmuneinfiltratesinhumanaorticdissection |