Non-invasive in situ visualization of the murine cranial vasculature

Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels,...

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Detalles Bibliográficos
Autores principales: Rosenblum, Jared S., Cappadona, Anthony J., Lookian, Pashayar P., Chandrashekhar, Vikram, Bryant, Jean-Paul, Chandrashekhar, Vibhu, Zhao, David Y., Knutsen, Russell H., Donahue, Danielle R., McGavern, Dorian B., Kozel, Beth A., Heiss, John D., Pacak, Karel, Zhuang, Zhengping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967186/
https://www.ncbi.nlm.nih.gov/pubmed/35373177
http://dx.doi.org/10.1016/j.crmeth.2021.100151
Descripción
Sumario:Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels, iterative sample processing and micro-computed tomography, and automatic deformable image registration, feature extraction, and visualization. This methodology is applicable to any tissue and allows rapid exploration of normal and altered pathologic states.

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