Non-invasive in situ visualization of the murine cranial vasculature

Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels,...

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Autores principales: Rosenblum, Jared S., Cappadona, Anthony J., Lookian, Pashayar P., Chandrashekhar, Vikram, Bryant, Jean-Paul, Chandrashekhar, Vibhu, Zhao, David Y., Knutsen, Russell H., Donahue, Danielle R., McGavern, Dorian B., Kozel, Beth A., Heiss, John D., Pacak, Karel, Zhuang, Zhengping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967186/
https://www.ncbi.nlm.nih.gov/pubmed/35373177
http://dx.doi.org/10.1016/j.crmeth.2021.100151
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author Rosenblum, Jared S.
Cappadona, Anthony J.
Lookian, Pashayar P.
Chandrashekhar, Vikram
Bryant, Jean-Paul
Chandrashekhar, Vibhu
Zhao, David Y.
Knutsen, Russell H.
Donahue, Danielle R.
McGavern, Dorian B.
Kozel, Beth A.
Heiss, John D.
Pacak, Karel
Zhuang, Zhengping
author_facet Rosenblum, Jared S.
Cappadona, Anthony J.
Lookian, Pashayar P.
Chandrashekhar, Vikram
Bryant, Jean-Paul
Chandrashekhar, Vibhu
Zhao, David Y.
Knutsen, Russell H.
Donahue, Danielle R.
McGavern, Dorian B.
Kozel, Beth A.
Heiss, John D.
Pacak, Karel
Zhuang, Zhengping
author_sort Rosenblum, Jared S.
collection PubMed
description Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels, iterative sample processing and micro-computed tomography, and automatic deformable image registration, feature extraction, and visualization. This methodology is applicable to any tissue and allows rapid exploration of normal and altered pathologic states.
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spelling pubmed-89671862022-04-25 Non-invasive in situ visualization of the murine cranial vasculature Rosenblum, Jared S. Cappadona, Anthony J. Lookian, Pashayar P. Chandrashekhar, Vikram Bryant, Jean-Paul Chandrashekhar, Vibhu Zhao, David Y. Knutsen, Russell H. Donahue, Danielle R. McGavern, Dorian B. Kozel, Beth A. Heiss, John D. Pacak, Karel Zhuang, Zhengping Cell Rep Methods Report Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire in situ cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels, iterative sample processing and micro-computed tomography, and automatic deformable image registration, feature extraction, and visualization. This methodology is applicable to any tissue and allows rapid exploration of normal and altered pathologic states. Elsevier 2022-01-24 /pmc/articles/PMC8967186/ /pubmed/35373177 http://dx.doi.org/10.1016/j.crmeth.2021.100151 Text en © 2021. https://creativecommons.org/licenses/by-nc-nd/3.0/igo/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/igo/).
spellingShingle Report
Rosenblum, Jared S.
Cappadona, Anthony J.
Lookian, Pashayar P.
Chandrashekhar, Vikram
Bryant, Jean-Paul
Chandrashekhar, Vibhu
Zhao, David Y.
Knutsen, Russell H.
Donahue, Danielle R.
McGavern, Dorian B.
Kozel, Beth A.
Heiss, John D.
Pacak, Karel
Zhuang, Zhengping
Non-invasive in situ visualization of the murine cranial vasculature
title Non-invasive in situ visualization of the murine cranial vasculature
title_full Non-invasive in situ visualization of the murine cranial vasculature
title_fullStr Non-invasive in situ visualization of the murine cranial vasculature
title_full_unstemmed Non-invasive in situ visualization of the murine cranial vasculature
title_short Non-invasive in situ visualization of the murine cranial vasculature
title_sort non-invasive in situ visualization of the murine cranial vasculature
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967186/
https://www.ncbi.nlm.nih.gov/pubmed/35373177
http://dx.doi.org/10.1016/j.crmeth.2021.100151
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