Cargando…

Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?

PURPOSE: To investigate the relationship between the microbiome of the female genital tract and endometriosis. METHODS: This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not hav...

Descripción completa

Detalles Bibliográficos
Autores principales: Oishi, Sugiko, Mekaru, Keiko, Tanaka, Suguru E., Arai, Wataru, Ashikawa, Kyota, Sakuraba, Yoshiyuki, Nishioka, Mikiko, Nakamura, Rie, Miyagi, Maho, Akamine, Kozue, Aoki, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967307/
https://www.ncbi.nlm.nih.gov/pubmed/35386386
http://dx.doi.org/10.1002/rmb2.12441
_version_ 1784678813954736128
author Oishi, Sugiko
Mekaru, Keiko
Tanaka, Suguru E.
Arai, Wataru
Ashikawa, Kyota
Sakuraba, Yoshiyuki
Nishioka, Mikiko
Nakamura, Rie
Miyagi, Maho
Akamine, Kozue
Aoki, Yoichi
author_facet Oishi, Sugiko
Mekaru, Keiko
Tanaka, Suguru E.
Arai, Wataru
Ashikawa, Kyota
Sakuraba, Yoshiyuki
Nishioka, Mikiko
Nakamura, Rie
Miyagi, Maho
Akamine, Kozue
Aoki, Yoichi
author_sort Oishi, Sugiko
collection PubMed
description PURPOSE: To investigate the relationship between the microbiome of the female genital tract and endometriosis. METHODS: This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next‐generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome. RESULTS: Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly (p = 0.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly (p = 0.02). CONCLUSION: Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis.
format Online
Article
Text
id pubmed-8967307
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-89673072022-04-05 Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid? Oishi, Sugiko Mekaru, Keiko Tanaka, Suguru E. Arai, Wataru Ashikawa, Kyota Sakuraba, Yoshiyuki Nishioka, Mikiko Nakamura, Rie Miyagi, Maho Akamine, Kozue Aoki, Yoichi Reprod Med Biol Original Article PURPOSE: To investigate the relationship between the microbiome of the female genital tract and endometriosis. METHODS: This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next‐generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome. RESULTS: Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly (p = 0.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly (p = 0.02). CONCLUSION: Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis. John Wiley and Sons Inc. 2022-01-29 /pmc/articles/PMC8967307/ /pubmed/35386386 http://dx.doi.org/10.1002/rmb2.12441 Text en © 2022 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Oishi, Sugiko
Mekaru, Keiko
Tanaka, Suguru E.
Arai, Wataru
Ashikawa, Kyota
Sakuraba, Yoshiyuki
Nishioka, Mikiko
Nakamura, Rie
Miyagi, Maho
Akamine, Kozue
Aoki, Yoichi
Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
title Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
title_full Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
title_fullStr Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
title_full_unstemmed Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
title_short Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
title_sort microbiome analysis in women with endometriosis: does a microbiome exist in peritoneal fluid and ovarian cystic fluid?
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967307/
https://www.ncbi.nlm.nih.gov/pubmed/35386386
http://dx.doi.org/10.1002/rmb2.12441
work_keys_str_mv AT oishisugiko microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT mekarukeiko microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT tanakasugurue microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT araiwataru microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT ashikawakyota microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT sakurabayoshiyuki microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT nishiokamikiko microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT nakamurarie microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT miyagimaho microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT akaminekozue microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid
AT aokiyoichi microbiomeanalysisinwomenwithendometriosisdoesamicrobiomeexistinperitonealfluidandovariancysticfluid