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A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)

The plateau zokor (Myospalax baileyi) is a native species to the Qinghai-Tibetan Plateau, inhabiting hypoxia and hypercapnia sealed subterranean burrows that pose several unique physiological challenges. In this study, we observed a novel heme-containing protein in the pulmonary surfactant (PS) of p...

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Autores principales: Li, Jimei, An, Zhifang, Wei, Linna, Xu, Bo, Wang, Zhijie, Gao, Conghui, Wei, Lian, Qi, Delin, Shi, Peng, Zhang, Tongzuo, Wei, Dengbang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967358/
https://www.ncbi.nlm.nih.gov/pubmed/35368669
http://dx.doi.org/10.3389/fgene.2022.824049
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author Li, Jimei
An, Zhifang
Wei, Linna
Xu, Bo
Wang, Zhijie
Gao, Conghui
Wei, Lian
Qi, Delin
Shi, Peng
Zhang, Tongzuo
Wei, Dengbang
author_facet Li, Jimei
An, Zhifang
Wei, Linna
Xu, Bo
Wang, Zhijie
Gao, Conghui
Wei, Lian
Qi, Delin
Shi, Peng
Zhang, Tongzuo
Wei, Dengbang
author_sort Li, Jimei
collection PubMed
description The plateau zokor (Myospalax baileyi) is a native species to the Qinghai-Tibetan Plateau, inhabiting hypoxia and hypercapnia sealed subterranean burrows that pose several unique physiological challenges. In this study, we observed a novel heme-containing protein in the pulmonary surfactant (PS) of plateau zokor, identified the encoding gene of the protein, predicted its origination and structure, verified its expression in alveolar epithelial cells, and determined the protein’s affinity to oxygen and its effect on the oxygen-dissolving capability in the PS of plateau zokors. The protein is an unusual homotetramer hemoglobin consisting of four γ-like subunits, and the subunit is encoded by a paralog gene of γ, that is γ-like. The divergence time of γ-like from γ is estimated by the molecular clock to be about 2.45 Mya. The generation of γ-like in plateau zokors might well relate to long-time stress of the high land hypoxia. Unlike γ, the γ-like has a hypoxia response element (HRE) and a lung tissue-specific enhancer in its upstream region, and it is expressed specifically in lung tissues and up-regulated by hypoxia. The protein is named as γ(4)-like which is expressed specifically in Alveolar epithelial type II (ATII) cells and secreted into the alveolar cavities through the osmiophilic multilamellar body (LBs). The γ(4)-like has a higher affinity to oxygen, and that increases significantly oxygen-dissolving capability in the PS of plateau zokors by its oxygenation function, which might be beneficial for the plateau zokors to obtain oxygen from the severe hypoxia environments by facilitating oxygen diffusion from alveoli to blood.
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spelling pubmed-89673582022-03-31 A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi) Li, Jimei An, Zhifang Wei, Linna Xu, Bo Wang, Zhijie Gao, Conghui Wei, Lian Qi, Delin Shi, Peng Zhang, Tongzuo Wei, Dengbang Front Genet Genetics The plateau zokor (Myospalax baileyi) is a native species to the Qinghai-Tibetan Plateau, inhabiting hypoxia and hypercapnia sealed subterranean burrows that pose several unique physiological challenges. In this study, we observed a novel heme-containing protein in the pulmonary surfactant (PS) of plateau zokor, identified the encoding gene of the protein, predicted its origination and structure, verified its expression in alveolar epithelial cells, and determined the protein’s affinity to oxygen and its effect on the oxygen-dissolving capability in the PS of plateau zokors. The protein is an unusual homotetramer hemoglobin consisting of four γ-like subunits, and the subunit is encoded by a paralog gene of γ, that is γ-like. The divergence time of γ-like from γ is estimated by the molecular clock to be about 2.45 Mya. The generation of γ-like in plateau zokors might well relate to long-time stress of the high land hypoxia. Unlike γ, the γ-like has a hypoxia response element (HRE) and a lung tissue-specific enhancer in its upstream region, and it is expressed specifically in lung tissues and up-regulated by hypoxia. The protein is named as γ(4)-like which is expressed specifically in Alveolar epithelial type II (ATII) cells and secreted into the alveolar cavities through the osmiophilic multilamellar body (LBs). The γ(4)-like has a higher affinity to oxygen, and that increases significantly oxygen-dissolving capability in the PS of plateau zokors by its oxygenation function, which might be beneficial for the plateau zokors to obtain oxygen from the severe hypoxia environments by facilitating oxygen diffusion from alveoli to blood. Frontiers Media S.A. 2022-03-15 /pmc/articles/PMC8967358/ /pubmed/35368669 http://dx.doi.org/10.3389/fgene.2022.824049 Text en Copyright © 2022 Li, An, Wei, Xu, Wang, Gao, Wei, Qi, Shi, Zhang and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Li, Jimei
An, Zhifang
Wei, Linna
Xu, Bo
Wang, Zhijie
Gao, Conghui
Wei, Lian
Qi, Delin
Shi, Peng
Zhang, Tongzuo
Wei, Dengbang
A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
title A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
title_full A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
title_fullStr A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
title_full_unstemmed A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
title_short A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi)
title_sort new homotetramer hemoglobin in the pulmonary surfactant of plateau zokors (myospalax baileyi)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967358/
https://www.ncbi.nlm.nih.gov/pubmed/35368669
http://dx.doi.org/10.3389/fgene.2022.824049
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