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The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study

PURPOSE: Lung cancer relapse may be associated with the presence of a small population of cancer stem cells (CSCs) with unlimited proliferative potential. Our study assessed the relationship between CSCs and the relapse rate in patients harboring adenocarcinoma (ADL) and squamous cell carcinoma of t...

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Autores principales: Masciale, Valentina, Banchelli, Federico, Grisendi, Giulia, D’Amico, Roberto, Maiorana, Antonino, Stefani, Alessandro, Morandi, Uliano, Stella, Franco, Dominici, Massimo, Aramini, Beatrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968653/
https://www.ncbi.nlm.nih.gov/pubmed/35356974
http://dx.doi.org/10.1093/stcltm/szab029
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author Masciale, Valentina
Banchelli, Federico
Grisendi, Giulia
D’Amico, Roberto
Maiorana, Antonino
Stefani, Alessandro
Morandi, Uliano
Stella, Franco
Dominici, Massimo
Aramini, Beatrice
author_facet Masciale, Valentina
Banchelli, Federico
Grisendi, Giulia
D’Amico, Roberto
Maiorana, Antonino
Stefani, Alessandro
Morandi, Uliano
Stella, Franco
Dominici, Massimo
Aramini, Beatrice
author_sort Masciale, Valentina
collection PubMed
description PURPOSE: Lung cancer relapse may be associated with the presence of a small population of cancer stem cells (CSCs) with unlimited proliferative potential. Our study assessed the relationship between CSCs and the relapse rate in patients harboring adenocarcinoma (ADL) and squamous cell carcinoma of the lung (SCCL). EXPERIMENTAL DESIGN: This is an observational prospective cohort study (NCT04634630) assessing the influence of CSC frequency on relapse rate after major lung resection in 35 patients harboring early (I-II) (n = 21) and locally advanced (IIIA) (n = 14) ADL and SCCL. There was a 2-year enrollment period followed by a 1-year follow-up period. Surgical tumor specimens were processed, and CSCs were quantified by cytofluorimetric analysis. RESULTS: Cancer stem cells were expressed in all patients with a median of 3.1% of the primary cell culture. Primary analysis showed no influence of CSC frequency on the risk of relapse (hazard ratio [HR] = 1.05, 95% confidence interval [CI] = 0.85-1.30). At secondary analysis, patients with locally advanced disease with higher CSC frequency had an increased risk of relapse (HR = 1.26, 95% CI = 1.14-1.39), whereas this was not observed in early-stage patients (HR = 0.90, 95% CI = 0.65-1.25). CONCLUSION: No association was found between CSC and relapse rates after major lung resection in patients harboring ACL and SCCL. However, in locally advanced-stage patients, a positive correlation was observed between CSC frequency and risk of relapse. These results indicate a need for further molecular investigations into the prognostic role of CSCs at different lung cancer stages. CLINICAL TRIAL REGISTRATION: NCT04634630.
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spelling pubmed-89686532022-03-31 The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study Masciale, Valentina Banchelli, Federico Grisendi, Giulia D’Amico, Roberto Maiorana, Antonino Stefani, Alessandro Morandi, Uliano Stella, Franco Dominici, Massimo Aramini, Beatrice Stem Cells Transl Med Human Clinical Article PURPOSE: Lung cancer relapse may be associated with the presence of a small population of cancer stem cells (CSCs) with unlimited proliferative potential. Our study assessed the relationship between CSCs and the relapse rate in patients harboring adenocarcinoma (ADL) and squamous cell carcinoma of the lung (SCCL). EXPERIMENTAL DESIGN: This is an observational prospective cohort study (NCT04634630) assessing the influence of CSC frequency on relapse rate after major lung resection in 35 patients harboring early (I-II) (n = 21) and locally advanced (IIIA) (n = 14) ADL and SCCL. There was a 2-year enrollment period followed by a 1-year follow-up period. Surgical tumor specimens were processed, and CSCs were quantified by cytofluorimetric analysis. RESULTS: Cancer stem cells were expressed in all patients with a median of 3.1% of the primary cell culture. Primary analysis showed no influence of CSC frequency on the risk of relapse (hazard ratio [HR] = 1.05, 95% confidence interval [CI] = 0.85-1.30). At secondary analysis, patients with locally advanced disease with higher CSC frequency had an increased risk of relapse (HR = 1.26, 95% CI = 1.14-1.39), whereas this was not observed in early-stage patients (HR = 0.90, 95% CI = 0.65-1.25). CONCLUSION: No association was found between CSC and relapse rates after major lung resection in patients harboring ACL and SCCL. However, in locally advanced-stage patients, a positive correlation was observed between CSC frequency and risk of relapse. These results indicate a need for further molecular investigations into the prognostic role of CSCs at different lung cancer stages. CLINICAL TRIAL REGISTRATION: NCT04634630. Oxford University Press 2022-02-23 /pmc/articles/PMC8968653/ /pubmed/35356974 http://dx.doi.org/10.1093/stcltm/szab029 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Human Clinical Article
Masciale, Valentina
Banchelli, Federico
Grisendi, Giulia
D’Amico, Roberto
Maiorana, Antonino
Stefani, Alessandro
Morandi, Uliano
Stella, Franco
Dominici, Massimo
Aramini, Beatrice
The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study
title The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study
title_full The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study
title_fullStr The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study
title_full_unstemmed The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study
title_short The Influence of Cancer Stem Cells on the Risk of Relapse in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Prospective Cohort Study
title_sort influence of cancer stem cells on the risk of relapse in adenocarcinoma and squamous cell carcinoma of the lung: a prospective cohort study
topic Human Clinical Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968653/
https://www.ncbi.nlm.nih.gov/pubmed/35356974
http://dx.doi.org/10.1093/stcltm/szab029
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