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Prokaryotic Collagen-Like Proteins as Novel Biomaterials

Collagens are the major structural component in animal extracellular matrices and are critical signaling molecules in various cell-matrix interactions. Its unique triple helical structure is enabled by tripeptide Gly-X-Y repeats. Understanding of sequence requirements for animal-derived collagen led...

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Autores principales: Picker, Jonathan, Lan, Ziyang, Arora, Srishtee, Green, Mykel, Hahn, Mariah, Cosgriff-Hernandez, Elizabeth, Hook, Magnus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968730/
https://www.ncbi.nlm.nih.gov/pubmed/35372322
http://dx.doi.org/10.3389/fbioe.2022.840939
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author Picker, Jonathan
Lan, Ziyang
Arora, Srishtee
Green, Mykel
Hahn, Mariah
Cosgriff-Hernandez, Elizabeth
Hook, Magnus
author_facet Picker, Jonathan
Lan, Ziyang
Arora, Srishtee
Green, Mykel
Hahn, Mariah
Cosgriff-Hernandez, Elizabeth
Hook, Magnus
author_sort Picker, Jonathan
collection PubMed
description Collagens are the major structural component in animal extracellular matrices and are critical signaling molecules in various cell-matrix interactions. Its unique triple helical structure is enabled by tripeptide Gly-X-Y repeats. Understanding of sequence requirements for animal-derived collagen led to the discovery of prokaryotic collagen-like protein in the early 2000s. These prokaryotic collagen-like proteins are structurally similar to mammalian collagens in many ways. However, unlike the challenges associated with recombinant expression of mammalian collagens, these prokaryotic collagen-like proteins can be readily expressed in E. coli and are amenable to genetic modification. In this review article, we will first discuss the properties of mammalian collagen and provide a comparative analysis of mammalian collagen and prokaryotic collagen-like proteins. We will then review the use of prokaryotic collagen-like proteins to both study the biology of conventional collagen and develop a new biomaterial platform. Finally, we will describe the application of Scl2 protein, a streptococcal collagen-like protein, in thromboresistant coating for cardiovascular devices, scaffolds for bone regeneration, chronic wound dressing and matrices for cartilage regeneration.
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spelling pubmed-89687302022-04-01 Prokaryotic Collagen-Like Proteins as Novel Biomaterials Picker, Jonathan Lan, Ziyang Arora, Srishtee Green, Mykel Hahn, Mariah Cosgriff-Hernandez, Elizabeth Hook, Magnus Front Bioeng Biotechnol Bioengineering and Biotechnology Collagens are the major structural component in animal extracellular matrices and are critical signaling molecules in various cell-matrix interactions. Its unique triple helical structure is enabled by tripeptide Gly-X-Y repeats. Understanding of sequence requirements for animal-derived collagen led to the discovery of prokaryotic collagen-like protein in the early 2000s. These prokaryotic collagen-like proteins are structurally similar to mammalian collagens in many ways. However, unlike the challenges associated with recombinant expression of mammalian collagens, these prokaryotic collagen-like proteins can be readily expressed in E. coli and are amenable to genetic modification. In this review article, we will first discuss the properties of mammalian collagen and provide a comparative analysis of mammalian collagen and prokaryotic collagen-like proteins. We will then review the use of prokaryotic collagen-like proteins to both study the biology of conventional collagen and develop a new biomaterial platform. Finally, we will describe the application of Scl2 protein, a streptococcal collagen-like protein, in thromboresistant coating for cardiovascular devices, scaffolds for bone regeneration, chronic wound dressing and matrices for cartilage regeneration. Frontiers Media S.A. 2022-03-17 /pmc/articles/PMC8968730/ /pubmed/35372322 http://dx.doi.org/10.3389/fbioe.2022.840939 Text en Copyright © 2022 Picker, Lan, Arora, Green, Hahn, Cosgriff-Hernandez and Hook. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Picker, Jonathan
Lan, Ziyang
Arora, Srishtee
Green, Mykel
Hahn, Mariah
Cosgriff-Hernandez, Elizabeth
Hook, Magnus
Prokaryotic Collagen-Like Proteins as Novel Biomaterials
title Prokaryotic Collagen-Like Proteins as Novel Biomaterials
title_full Prokaryotic Collagen-Like Proteins as Novel Biomaterials
title_fullStr Prokaryotic Collagen-Like Proteins as Novel Biomaterials
title_full_unstemmed Prokaryotic Collagen-Like Proteins as Novel Biomaterials
title_short Prokaryotic Collagen-Like Proteins as Novel Biomaterials
title_sort prokaryotic collagen-like proteins as novel biomaterials
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968730/
https://www.ncbi.nlm.nih.gov/pubmed/35372322
http://dx.doi.org/10.3389/fbioe.2022.840939
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