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A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways

As a key regulator of gene transcription and post-transcriptional modification, miRNAs play a wide range of roles in skeletal muscle development. Skeletal muscle satellite cells contribute to postnatal growing muscle fibers. Thus, the goal of this study was to explore the effects of novel miRNA Y-56...

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Autores principales: Song, Jie, Hao, Linlin, Zeng, Xiangfang, Yang, Rui, Qiao, Shiyan, Wang, Chunli, Yu, Hao, Wang, Siyao, Jiao, Yingying, Jia, Hongyao, Liu, Songcai, Zhang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968951/
https://www.ncbi.nlm.nih.gov/pubmed/35372530
http://dx.doi.org/10.3389/fvets.2022.754435
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author Song, Jie
Hao, Linlin
Zeng, Xiangfang
Yang, Rui
Qiao, Shiyan
Wang, Chunli
Yu, Hao
Wang, Siyao
Jiao, Yingying
Jia, Hongyao
Liu, Songcai
Zhang, Ying
author_facet Song, Jie
Hao, Linlin
Zeng, Xiangfang
Yang, Rui
Qiao, Shiyan
Wang, Chunli
Yu, Hao
Wang, Siyao
Jiao, Yingying
Jia, Hongyao
Liu, Songcai
Zhang, Ying
author_sort Song, Jie
collection PubMed
description As a key regulator of gene transcription and post-transcriptional modification, miRNAs play a wide range of roles in skeletal muscle development. Skeletal muscle satellite cells contribute to postnatal growing muscle fibers. Thus, the goal of this study was to explore the effects of novel miRNA Y-56 on porcine skeletal muscle satellite cells (PSCs). We found that Y-56 was highly expressed in porcine muscle tissues, and its expression was higher in Bama Xiang pigs than in Landrace pigs. The EdU assay, cell counting kit-8, and flow cytometry results showed that Y-56 overexpression suppressed cell proliferation and cell cycle, whereas Y-56 inhibition resulted in the opposite consequences. The results of qRT-PCR and Western blot showed that Y-56 remarkably inhibited the expression levels of cyclin-dependent kinase 4 (CDK4), proliferating cell nuclear antigen (PCNA), and cyclin D1. We identified that IGF-1R was a direct target of Y-56 by dual-luciferase reporter assay. Moreover, IGF-1R overexpression promoted the proliferation and cell cycle process of PSCs and upregulated the expression of CDK4, PCNA, and cyclin D1. Conversely, IGF-1R knockdown had the opposite effect. Furthermore, IGF-1R overexpression partially reversed the inhibition of the cell proliferation and cell cycle process of PSCs and the downregulation of the expression of CDK4, PCNA, and Cyclin D1 caused by Y-56 overexpression. Finally, Y-56 inhibited the protein expression levels of p-AKT and p-ERK. Collectively, our findings suggested that Y-56 represses the proliferation and cell cycle process of PSCs by targeting IGF-1R-mediated AKT and ERK pathways.
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spelling pubmed-89689512022-04-01 A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways Song, Jie Hao, Linlin Zeng, Xiangfang Yang, Rui Qiao, Shiyan Wang, Chunli Yu, Hao Wang, Siyao Jiao, Yingying Jia, Hongyao Liu, Songcai Zhang, Ying Front Vet Sci Veterinary Science As a key regulator of gene transcription and post-transcriptional modification, miRNAs play a wide range of roles in skeletal muscle development. Skeletal muscle satellite cells contribute to postnatal growing muscle fibers. Thus, the goal of this study was to explore the effects of novel miRNA Y-56 on porcine skeletal muscle satellite cells (PSCs). We found that Y-56 was highly expressed in porcine muscle tissues, and its expression was higher in Bama Xiang pigs than in Landrace pigs. The EdU assay, cell counting kit-8, and flow cytometry results showed that Y-56 overexpression suppressed cell proliferation and cell cycle, whereas Y-56 inhibition resulted in the opposite consequences. The results of qRT-PCR and Western blot showed that Y-56 remarkably inhibited the expression levels of cyclin-dependent kinase 4 (CDK4), proliferating cell nuclear antigen (PCNA), and cyclin D1. We identified that IGF-1R was a direct target of Y-56 by dual-luciferase reporter assay. Moreover, IGF-1R overexpression promoted the proliferation and cell cycle process of PSCs and upregulated the expression of CDK4, PCNA, and cyclin D1. Conversely, IGF-1R knockdown had the opposite effect. Furthermore, IGF-1R overexpression partially reversed the inhibition of the cell proliferation and cell cycle process of PSCs and the downregulation of the expression of CDK4, PCNA, and Cyclin D1 caused by Y-56 overexpression. Finally, Y-56 inhibited the protein expression levels of p-AKT and p-ERK. Collectively, our findings suggested that Y-56 represses the proliferation and cell cycle process of PSCs by targeting IGF-1R-mediated AKT and ERK pathways. Frontiers Media S.A. 2022-03-17 /pmc/articles/PMC8968951/ /pubmed/35372530 http://dx.doi.org/10.3389/fvets.2022.754435 Text en Copyright © 2022 Song, Hao, Zeng, Yang, Qiao, Wang, Yu, Wang, Jiao, Jia, Liu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Song, Jie
Hao, Linlin
Zeng, Xiangfang
Yang, Rui
Qiao, Shiyan
Wang, Chunli
Yu, Hao
Wang, Siyao
Jiao, Yingying
Jia, Hongyao
Liu, Songcai
Zhang, Ying
A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways
title A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways
title_full A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways
title_fullStr A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways
title_full_unstemmed A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways
title_short A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways
title_sort novel mirna y-56 targeting igf-1r mediates the proliferation of porcine skeletal muscle satellite cells through akt and erk pathways
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968951/
https://www.ncbi.nlm.nih.gov/pubmed/35372530
http://dx.doi.org/10.3389/fvets.2022.754435
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