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Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

BACKGROUND: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. RESULTS: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem bor...

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Autores principales: Xia, Yi-Han, Ding, Bao-Jian, Dong, Shuang-Lin, Wang, Hong-Lei, Hofvander, Per, Löfstedt, Christer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969271/
https://www.ncbi.nlm.nih.gov/pubmed/35361182
http://dx.doi.org/10.1186/s12915-022-01281-8
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author Xia, Yi-Han
Ding, Bao-Jian
Dong, Shuang-Lin
Wang, Hong-Lei
Hofvander, Per
Löfstedt, Christer
author_facet Xia, Yi-Han
Ding, Bao-Jian
Dong, Shuang-Lin
Wang, Hong-Lei
Hofvander, Per
Löfstedt, Christer
author_sort Xia, Yi-Han
collection PubMed
description BACKGROUND: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. RESULTS: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. CONCLUSIONS: Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01281-8.
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spelling pubmed-89692712022-04-01 Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes Xia, Yi-Han Ding, Bao-Jian Dong, Shuang-Lin Wang, Hong-Lei Hofvander, Per Löfstedt, Christer BMC Biol Research Article BACKGROUND: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. RESULTS: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. CONCLUSIONS: Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01281-8. BioMed Central 2022-03-31 /pmc/articles/PMC8969271/ /pubmed/35361182 http://dx.doi.org/10.1186/s12915-022-01281-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Xia, Yi-Han
Ding, Bao-Jian
Dong, Shuang-Lin
Wang, Hong-Lei
Hofvander, Per
Löfstedt, Christer
Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
title Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
title_full Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
title_fullStr Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
title_full_unstemmed Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
title_short Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
title_sort release of moth pheromone compounds from nicotiana benthamiana upon transient expression of heterologous biosynthetic genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969271/
https://www.ncbi.nlm.nih.gov/pubmed/35361182
http://dx.doi.org/10.1186/s12915-022-01281-8
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