Prime-seq, efficient and powerful bulk RNA sequencing

Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost...

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Detalles Bibliográficos
Autores principales: Janjic, Aleksandar, Wange, Lucas E., Bagnoli, Johannes W., Geuder, Johanna, Nguyen, Phong, Richter, Daniel, Vieth, Beate, Vick, Binje, Jeremias, Irmela, Ziegenhain, Christoph, Hellmann, Ines, Enard, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969310/
https://www.ncbi.nlm.nih.gov/pubmed/35361256
http://dx.doi.org/10.1186/s13059-022-02660-8

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