Cargando…

Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence

In the processes that give rise to new species, changes first occur at the population level. But with the continuous nature of the divergence process, change in biological properties delimiting the shift from “individuals of divergent populations” towards “individuals of distinct species”, as well a...

Descripción completa

Detalles Bibliográficos
Autores principales: Rasolonjatovo, Safidy M., Scherz, Mark D., Schmidt, Robin, Glos, Julian, Rakotoarison, Andolalao, Raselimanana, Achille P., Vences, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8970393/
https://www.ncbi.nlm.nih.gov/pubmed/35358210
http://dx.doi.org/10.1371/journal.pone.0263764
_version_ 1784679450595557376
author Rasolonjatovo, Safidy M.
Scherz, Mark D.
Schmidt, Robin
Glos, Julian
Rakotoarison, Andolalao
Raselimanana, Achille P.
Vences, Miguel
author_facet Rasolonjatovo, Safidy M.
Scherz, Mark D.
Schmidt, Robin
Glos, Julian
Rakotoarison, Andolalao
Raselimanana, Achille P.
Vences, Miguel
author_sort Rasolonjatovo, Safidy M.
collection PubMed
description In the processes that give rise to new species, changes first occur at the population level. But with the continuous nature of the divergence process, change in biological properties delimiting the shift from “individuals of divergent populations” towards “individuals of distinct species”, as well as abiotic factors driving the change, remain largely ambivalent. Here we study diversification processes at the population level in a semi-aquatic frog, Mantidactylus (Brygoomantis) bellyi, across the diverse vegetation types of Montagne d’Ambre National Park (MANP), Madagascar. Genetic diversity was assessed with seven newly developed microsatellite markers as well as mitochondrial DNA sequences and concordance with patterns of ecological, morphological, and bioacoustic divergence evaluated. We found M. bellyi lacking mitochondrial differentiation within MANP, while microsatellite datasets partitioned them into three highly differentiated, geographically separated subpopulations (with indications for up to five subpopulations). The molecular grouping–primarily clustering individuals by geographic proximity–was coincident with differences in mean depth and width of waters, suggesting a possible role of fluvial characteristics in genetic exchange in this stream-breeding species. Genetic clustering not consistent with differences in call properties, except for dominant call frequencies under the two-subpopulations model. Morphological divergence was mostly consistent with the genetic clustering; subpopulations strongly differed by their snout-vent length, with individuals from high-elevation subpopulations smaller than those from populations below 1000 m above sea level. These results exemplify how mountains and environmental conditions might primarily shape genetic and morphological divergence in frog populations, without strongly affecting their calls.
format Online
Article
Text
id pubmed-8970393
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-89703932022-04-01 Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence Rasolonjatovo, Safidy M. Scherz, Mark D. Schmidt, Robin Glos, Julian Rakotoarison, Andolalao Raselimanana, Achille P. Vences, Miguel PLoS One Research Article In the processes that give rise to new species, changes first occur at the population level. But with the continuous nature of the divergence process, change in biological properties delimiting the shift from “individuals of divergent populations” towards “individuals of distinct species”, as well as abiotic factors driving the change, remain largely ambivalent. Here we study diversification processes at the population level in a semi-aquatic frog, Mantidactylus (Brygoomantis) bellyi, across the diverse vegetation types of Montagne d’Ambre National Park (MANP), Madagascar. Genetic diversity was assessed with seven newly developed microsatellite markers as well as mitochondrial DNA sequences and concordance with patterns of ecological, morphological, and bioacoustic divergence evaluated. We found M. bellyi lacking mitochondrial differentiation within MANP, while microsatellite datasets partitioned them into three highly differentiated, geographically separated subpopulations (with indications for up to five subpopulations). The molecular grouping–primarily clustering individuals by geographic proximity–was coincident with differences in mean depth and width of waters, suggesting a possible role of fluvial characteristics in genetic exchange in this stream-breeding species. Genetic clustering not consistent with differences in call properties, except for dominant call frequencies under the two-subpopulations model. Morphological divergence was mostly consistent with the genetic clustering; subpopulations strongly differed by their snout-vent length, with individuals from high-elevation subpopulations smaller than those from populations below 1000 m above sea level. These results exemplify how mountains and environmental conditions might primarily shape genetic and morphological divergence in frog populations, without strongly affecting their calls. Public Library of Science 2022-03-31 /pmc/articles/PMC8970393/ /pubmed/35358210 http://dx.doi.org/10.1371/journal.pone.0263764 Text en © 2022 Rasolonjatovo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rasolonjatovo, Safidy M.
Scherz, Mark D.
Schmidt, Robin
Glos, Julian
Rakotoarison, Andolalao
Raselimanana, Achille P.
Vences, Miguel
Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence
title Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence
title_full Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence
title_fullStr Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence
title_full_unstemmed Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence
title_short Population diversification in the frog Mantidactylus bellyi on an isolated massif in northern Madagascar based on genetic, morphological, bioacoustic and ecological evidence
title_sort population diversification in the frog mantidactylus bellyi on an isolated massif in northern madagascar based on genetic, morphological, bioacoustic and ecological evidence
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8970393/
https://www.ncbi.nlm.nih.gov/pubmed/35358210
http://dx.doi.org/10.1371/journal.pone.0263764
work_keys_str_mv AT rasolonjatovosafidym populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence
AT scherzmarkd populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence
AT schmidtrobin populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence
AT glosjulian populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence
AT rakotoarisonandolalao populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence
AT raselimananaachillep populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence
AT vencesmiguel populationdiversificationinthefrogmantidactylusbellyionanisolatedmassifinnorthernmadagascarbasedongeneticmorphologicalbioacousticandecologicalevidence