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Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis

Fluidic chips have attracted considerable interest in recent years for their potential applications in analytical devices. Previously, we developed a method to fabricate polydimethylsiloxane (PDMS) fluidic chips via templates made using a low-priced commercial Fused Deposition Modeling (FDM) type 3D...

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Autores principales: Yamashita, Tomohisa, Muramoto, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971176/
https://www.ncbi.nlm.nih.gov/pubmed/35286631
http://dx.doi.org/10.1007/s44211-022-00070-1
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author Yamashita, Tomohisa
Muramoto, Tatsuya
author_facet Yamashita, Tomohisa
Muramoto, Tatsuya
author_sort Yamashita, Tomohisa
collection PubMed
description Fluidic chips have attracted considerable interest in recent years for their potential applications in analytical devices. Previously, we developed a method to fabricate polydimethylsiloxane (PDMS) fluidic chips via templates made using a low-priced commercial Fused Deposition Modeling (FDM) type 3D printer and polymer coatings. However, in general, methods using a template cannot form a flow channel thinner than the template thickness and the width. In this study, the inner wall of a PDMS fluidic chip was coated with PDMS to create a chip with a channel inner diameter smaller than a template. Then, by measuring the flow signal of methyl orange with a single line, the basic properties of the non-coated and coated chip were investigated. As a result, almost the same flow profile was obtained in non-coated and coated chips at the same linear velocity and the same sample injection length. By coating and narrowing the channel width, it is possible to save the amount of sample and carrier solution. Measuring hydrazine in water using a coated chip was also tried. The calibration curve indicated good linearity in the range of 1–6 ppm. However, a concentration point of 7 ppm deviated. The reason for this deviation was presumably due to inadequate mixing of the sample and reagent. By decreasing the flow rate, the calibration curve indicated good linearity in the range of 1–7 ppm. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s44211-022-00070-1.
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spelling pubmed-89711762022-04-07 Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis Yamashita, Tomohisa Muramoto, Tatsuya Anal Sci Original Paper Fluidic chips have attracted considerable interest in recent years for their potential applications in analytical devices. Previously, we developed a method to fabricate polydimethylsiloxane (PDMS) fluidic chips via templates made using a low-priced commercial Fused Deposition Modeling (FDM) type 3D printer and polymer coatings. However, in general, methods using a template cannot form a flow channel thinner than the template thickness and the width. In this study, the inner wall of a PDMS fluidic chip was coated with PDMS to create a chip with a channel inner diameter smaller than a template. Then, by measuring the flow signal of methyl orange with a single line, the basic properties of the non-coated and coated chip were investigated. As a result, almost the same flow profile was obtained in non-coated and coated chips at the same linear velocity and the same sample injection length. By coating and narrowing the channel width, it is possible to save the amount of sample and carrier solution. Measuring hydrazine in water using a coated chip was also tried. The calibration curve indicated good linearity in the range of 1–6 ppm. However, a concentration point of 7 ppm deviated. The reason for this deviation was presumably due to inadequate mixing of the sample and reagent. By decreasing the flow rate, the calibration curve indicated good linearity in the range of 1–7 ppm. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s44211-022-00070-1. Springer Singapore 2022-02-15 2022 /pmc/articles/PMC8971176/ /pubmed/35286631 http://dx.doi.org/10.1007/s44211-022-00070-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Yamashita, Tomohisa
Muramoto, Tatsuya
Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis
title Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis
title_full Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis
title_fullStr Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis
title_full_unstemmed Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis
title_short Reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3D-printed sacrificial template and their application for flow-injection analysis
title_sort reducing the channel diameter of polydimethylsiloxane fluidic chips made by a 3d-printed sacrificial template and their application for flow-injection analysis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971176/
https://www.ncbi.nlm.nih.gov/pubmed/35286631
http://dx.doi.org/10.1007/s44211-022-00070-1
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