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Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome

The mechanisms underlining pathogenesis of polycystic ovary syndrome (PCOS) remain largely unknown. Dysfunction of ovarian granulosa cells plays an important role. The present study performed the lncRNA and mRNA profiling by whole genome transcriptomic sequencing of ovary granulosa cells from women...

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Autores principales: Deng, Yan, Li, Hu, Song, Yi, Cen, Juan, Zhang, Yuying, Sui, Yi, Cui, Dexuan, Li, Tin Chiu, Xu, Yan, Wang, Chi Chiu, Chung, Pui Wah Jacqueline, Tang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971550/
https://www.ncbi.nlm.nih.gov/pubmed/35370991
http://dx.doi.org/10.3389/fendo.2022.781149
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author Deng, Yan
Li, Hu
Song, Yi
Cen, Juan
Zhang, Yuying
Sui, Yi
Cui, Dexuan
Li, Tin Chiu
Xu, Yan
Wang, Chi Chiu
Chung, Pui Wah Jacqueline
Tang, Tao
author_facet Deng, Yan
Li, Hu
Song, Yi
Cen, Juan
Zhang, Yuying
Sui, Yi
Cui, Dexuan
Li, Tin Chiu
Xu, Yan
Wang, Chi Chiu
Chung, Pui Wah Jacqueline
Tang, Tao
author_sort Deng, Yan
collection PubMed
description The mechanisms underlining pathogenesis of polycystic ovary syndrome (PCOS) remain largely unknown. Dysfunction of ovarian granulosa cells plays an important role. The present study performed the lncRNA and mRNA profiling by whole genome transcriptomic sequencing of ovary granulosa cells from women with PCOS and investigated the potential role of differentially expressed gens (DEGs) in the pathomechanism of PCOS. In total, 1,936 DEGs (30 upregulated and 1,906 downregulated mRNAs and lncRNAs) were identified in the ovary granulosa cells between control and PCOS group. Functional enrichment analysis showed that DEGs were mainly associated with cytokine–cytokine receptor interaction, neuroactive ligand–receptor interaction, and olfactory transduction. qRT-PCR validated the upregulation of DLGAP5 mRNA in ovary from PCOS group when compared to control group. Immunostaining and TUNEL assays showed that DLGAP5 protein level was increased while apoptosis was decreased in follicles of ovary in PCOS group. In vitro functional assays showed that DLGPA5 knockdown repressed viability and proliferation, but enhanced apoptosis and disrupted cell cycle in granulosa cells; while DLGAP5 overexpression had the opposite effects in granulosa cells. In conclusion, the study showed differentially expressed lncRNA and mRNA profile in the granulosa cells in ovaries of PCOS. Functional results demonstrated that DLGAP5 is a dysregulated candidate gene in the pathogenesis of PCOS, especially granulosa cell apoptosis and proliferation.
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spelling pubmed-89715502022-04-02 Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome Deng, Yan Li, Hu Song, Yi Cen, Juan Zhang, Yuying Sui, Yi Cui, Dexuan Li, Tin Chiu Xu, Yan Wang, Chi Chiu Chung, Pui Wah Jacqueline Tang, Tao Front Endocrinol (Lausanne) Endocrinology The mechanisms underlining pathogenesis of polycystic ovary syndrome (PCOS) remain largely unknown. Dysfunction of ovarian granulosa cells plays an important role. The present study performed the lncRNA and mRNA profiling by whole genome transcriptomic sequencing of ovary granulosa cells from women with PCOS and investigated the potential role of differentially expressed gens (DEGs) in the pathomechanism of PCOS. In total, 1,936 DEGs (30 upregulated and 1,906 downregulated mRNAs and lncRNAs) were identified in the ovary granulosa cells between control and PCOS group. Functional enrichment analysis showed that DEGs were mainly associated with cytokine–cytokine receptor interaction, neuroactive ligand–receptor interaction, and olfactory transduction. qRT-PCR validated the upregulation of DLGAP5 mRNA in ovary from PCOS group when compared to control group. Immunostaining and TUNEL assays showed that DLGAP5 protein level was increased while apoptosis was decreased in follicles of ovary in PCOS group. In vitro functional assays showed that DLGPA5 knockdown repressed viability and proliferation, but enhanced apoptosis and disrupted cell cycle in granulosa cells; while DLGAP5 overexpression had the opposite effects in granulosa cells. In conclusion, the study showed differentially expressed lncRNA and mRNA profile in the granulosa cells in ovaries of PCOS. Functional results demonstrated that DLGAP5 is a dysregulated candidate gene in the pathogenesis of PCOS, especially granulosa cell apoptosis and proliferation. Frontiers Media S.A. 2022-03-18 /pmc/articles/PMC8971550/ /pubmed/35370991 http://dx.doi.org/10.3389/fendo.2022.781149 Text en Copyright © 2022 Deng, Li, Song, Cen, Zhang, Sui, Cui, Li, Xu, Wang, Chung and Tang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Deng, Yan
Li, Hu
Song, Yi
Cen, Juan
Zhang, Yuying
Sui, Yi
Cui, Dexuan
Li, Tin Chiu
Xu, Yan
Wang, Chi Chiu
Chung, Pui Wah Jacqueline
Tang, Tao
Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome
title Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome
title_full Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome
title_fullStr Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome
title_full_unstemmed Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome
title_short Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome
title_sort whole genome transcriptomic analysis of ovary granulosa cells revealed an anti-apoptosis regulatory gene dlgap5 in polycystic ovary syndrome
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971550/
https://www.ncbi.nlm.nih.gov/pubmed/35370991
http://dx.doi.org/10.3389/fendo.2022.781149
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