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Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice

Limb muscle dysfunction is a hallmark of Chronic Obstructive Pulmonary Disease (COPD) which is further worsened following a viral-induced acute exacerbation of COPD (AECOPD). An amplified airway inflammation underlies the aggravated respiratory symptoms seen during AECOPD, however, its contributory...

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Autores principales: Mou, Kevin, Chan, Stanley M. H., Brassington, Kurt, Dobric, Aleksandar, De Luca, Simone N., Seow, Huei Jiunn, Selemidis, Stavros, Bozinovski, Steven, Vlahos, Ross
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971713/
https://www.ncbi.nlm.nih.gov/pubmed/35370652
http://dx.doi.org/10.3389/fphar.2022.859146
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author Mou, Kevin
Chan, Stanley M. H.
Brassington, Kurt
Dobric, Aleksandar
De Luca, Simone N.
Seow, Huei Jiunn
Selemidis, Stavros
Bozinovski, Steven
Vlahos, Ross
author_facet Mou, Kevin
Chan, Stanley M. H.
Brassington, Kurt
Dobric, Aleksandar
De Luca, Simone N.
Seow, Huei Jiunn
Selemidis, Stavros
Bozinovski, Steven
Vlahos, Ross
author_sort Mou, Kevin
collection PubMed
description Limb muscle dysfunction is a hallmark of Chronic Obstructive Pulmonary Disease (COPD) which is further worsened following a viral-induced acute exacerbation of COPD (AECOPD). An amplified airway inflammation underlies the aggravated respiratory symptoms seen during AECOPD, however, its contributory role to limb muscle dysfunction is unclear. The present study examined the impact of influenza A virus (IAV)-induced exacerbation on hind limb muscle parameters. Airway inflammation was established in male BALB/c mice by exposure to cigarette smoke (CS) for 8 weeks. Exacerbation was then induced via inoculation with IAV, and various lung and muscle parameters were assessed on day 3 (peak of airway inflammation) and day 10 (resolution phase) post-infection. IAV infection exacerbated CS-induced airway inflammation as evidenced by further increases in immune cell counts within bronchoalveolar lavage fluid. Despite no significant impact on muscle mass, IAV exacerbation worsened the force-generating capacity of the tibialis anterior (TA) muscle. Protein oxidation and myogenic disruption was observed in the TA following CS exposure, however, IAV exacerbation did not augment these detrimental processes. To further explore the contributory role of airway inflammation on myogenic signaling, cultured myotubes were exposed to conditioned medium (CM) derived from bronchial epithelial cells stimulated with polyinosinic:polycytidylic acid and cigarette smoke extract (CSE). Despite an amplified inflammatory response in the lung epithelial cells, the CM derived from these cells did not potentiate myogenic disruption in the C2C12 myotubes. In conclusion, our data suggest that certain parameters of limb muscle dysfunction seen during viral-induced AECOPD may be independent of airway inflammation.
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spelling pubmed-89717132022-04-02 Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice Mou, Kevin Chan, Stanley M. H. Brassington, Kurt Dobric, Aleksandar De Luca, Simone N. Seow, Huei Jiunn Selemidis, Stavros Bozinovski, Steven Vlahos, Ross Front Pharmacol Pharmacology Limb muscle dysfunction is a hallmark of Chronic Obstructive Pulmonary Disease (COPD) which is further worsened following a viral-induced acute exacerbation of COPD (AECOPD). An amplified airway inflammation underlies the aggravated respiratory symptoms seen during AECOPD, however, its contributory role to limb muscle dysfunction is unclear. The present study examined the impact of influenza A virus (IAV)-induced exacerbation on hind limb muscle parameters. Airway inflammation was established in male BALB/c mice by exposure to cigarette smoke (CS) for 8 weeks. Exacerbation was then induced via inoculation with IAV, and various lung and muscle parameters were assessed on day 3 (peak of airway inflammation) and day 10 (resolution phase) post-infection. IAV infection exacerbated CS-induced airway inflammation as evidenced by further increases in immune cell counts within bronchoalveolar lavage fluid. Despite no significant impact on muscle mass, IAV exacerbation worsened the force-generating capacity of the tibialis anterior (TA) muscle. Protein oxidation and myogenic disruption was observed in the TA following CS exposure, however, IAV exacerbation did not augment these detrimental processes. To further explore the contributory role of airway inflammation on myogenic signaling, cultured myotubes were exposed to conditioned medium (CM) derived from bronchial epithelial cells stimulated with polyinosinic:polycytidylic acid and cigarette smoke extract (CSE). Despite an amplified inflammatory response in the lung epithelial cells, the CM derived from these cells did not potentiate myogenic disruption in the C2C12 myotubes. In conclusion, our data suggest that certain parameters of limb muscle dysfunction seen during viral-induced AECOPD may be independent of airway inflammation. Frontiers Media S.A. 2022-03-18 /pmc/articles/PMC8971713/ /pubmed/35370652 http://dx.doi.org/10.3389/fphar.2022.859146 Text en Copyright © 2022 Mou, Chan, Brassington, Dobric, De Luca, Seow, Selemidis, Bozinovski and Vlahos. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Mou, Kevin
Chan, Stanley M. H.
Brassington, Kurt
Dobric, Aleksandar
De Luca, Simone N.
Seow, Huei Jiunn
Selemidis, Stavros
Bozinovski, Steven
Vlahos, Ross
Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice
title Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice
title_full Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice
title_fullStr Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice
title_full_unstemmed Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice
title_short Influenza A Virus-Driven Airway Inflammation may be Dissociated From Limb Muscle Atrophy in Cigarette Smoke-Exposed Mice
title_sort influenza a virus-driven airway inflammation may be dissociated from limb muscle atrophy in cigarette smoke-exposed mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971713/
https://www.ncbi.nlm.nih.gov/pubmed/35370652
http://dx.doi.org/10.3389/fphar.2022.859146
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