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Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131

Extraintestinal pathogenic Escherichia coli (ExPEC) causes infections outside the intestine. Particular ExPEC clones, such as clonal complex (CC)/sequence type (ST)131, have been known to sequentially accumulate antimicrobial resistance that starts with chromosomal mutations against fluoroquinolones...

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Autores principales: Sanz, María Belén, De Belder, Denise, de Mendieta, JM, Faccone, Diego, Poklepovich, Tomás, Lucero, Celeste, Rapoport, Melina, Campos, Josefina, Tuduri, Ezequiel, Saavedra, Mathew O., Van der Ploeg, Claudia, Rogé, Ariel, Pasteran, Fernando, Corso, Alejandra, Rosato, Adriana E., Gomez, Sonia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971848/
https://www.ncbi.nlm.nih.gov/pubmed/35369469
http://dx.doi.org/10.3389/fmicb.2022.830209
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author Sanz, María Belén
De Belder, Denise
de Mendieta, JM
Faccone, Diego
Poklepovich, Tomás
Lucero, Celeste
Rapoport, Melina
Campos, Josefina
Tuduri, Ezequiel
Saavedra, Mathew O.
Van der Ploeg, Claudia
Rogé, Ariel
Pasteran, Fernando
Corso, Alejandra
Rosato, Adriana E.
Gomez, Sonia A.
author_facet Sanz, María Belén
De Belder, Denise
de Mendieta, JM
Faccone, Diego
Poklepovich, Tomás
Lucero, Celeste
Rapoport, Melina
Campos, Josefina
Tuduri, Ezequiel
Saavedra, Mathew O.
Van der Ploeg, Claudia
Rogé, Ariel
Pasteran, Fernando
Corso, Alejandra
Rosato, Adriana E.
Gomez, Sonia A.
author_sort Sanz, María Belén
collection PubMed
description Extraintestinal pathogenic Escherichia coli (ExPEC) causes infections outside the intestine. Particular ExPEC clones, such as clonal complex (CC)/sequence type (ST)131, have been known to sequentially accumulate antimicrobial resistance that starts with chromosomal mutations against fluoroquinolones, followed with the acquisition of bla(CTX–M–15) and, more recently, carbapenemases. Here we aimed to investigate the distribution of global epidemic clones of carbapenemase-producing ExPEC from Argentina in representative clinical isolates recovered between July 2008 and March 2017. Carbapenemase-producing ExPEC (n = 160) were referred to the Argentinean reference laboratory. Of these, 71 were selected for genome sequencing. Phenotypic and microbiological studies confirmed the presence of carbapenemases confirmed as KPC-2 (n = 52), NDM-1 (n = 16), IMP-8 (n = 2), and VIM-1 (n = 1) producers. The isolates had been recovered mainly from urine, blood, and abdominal fluids among others, and some were from screening samples. After analyzing the virulence gene content, 76% of the isolates were considered ExPEC, although non-ExPEC isolates were also obtained from extraintestinal sites. Pan-genome phylogeny and clonal analysis showed great clonal diversity, although the first phylogroup in abundance was phylogroup A, harboring CC10 isolates, followed by phylogroup B2 with CC/ST131, mostly H30Rx, the subclone co-producing CTX-M-15. Phylogroups D, B1, C, F, and E were also detected with fewer strains. CC10 and CC/ST131 were found throughout the country. In addition, CC10 nucleated most metalloenzymes, such as NDM-1. Other relevant international clones were identified, such as CC/ST38, CC155, CC14/ST1193, and CC23. Two isolates co-produced KPC-2 and OXA-163 or OXA-439, a point mutation variant of OXA-163, and three isolates co-produced MCR-1 among other resistance genes. To conclude, in this work, we described the molecular epidemiology of carbapenemase-producing ExPEC in Argentina. Further studies are necessary to determine the plasmid families disseminating carbapenemases in ExPEC in this region.
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spelling pubmed-89718482022-04-02 Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131 Sanz, María Belén De Belder, Denise de Mendieta, JM Faccone, Diego Poklepovich, Tomás Lucero, Celeste Rapoport, Melina Campos, Josefina Tuduri, Ezequiel Saavedra, Mathew O. Van der Ploeg, Claudia Rogé, Ariel Pasteran, Fernando Corso, Alejandra Rosato, Adriana E. Gomez, Sonia A. Front Microbiol Microbiology Extraintestinal pathogenic Escherichia coli (ExPEC) causes infections outside the intestine. Particular ExPEC clones, such as clonal complex (CC)/sequence type (ST)131, have been known to sequentially accumulate antimicrobial resistance that starts with chromosomal mutations against fluoroquinolones, followed with the acquisition of bla(CTX–M–15) and, more recently, carbapenemases. Here we aimed to investigate the distribution of global epidemic clones of carbapenemase-producing ExPEC from Argentina in representative clinical isolates recovered between July 2008 and March 2017. Carbapenemase-producing ExPEC (n = 160) were referred to the Argentinean reference laboratory. Of these, 71 were selected for genome sequencing. Phenotypic and microbiological studies confirmed the presence of carbapenemases confirmed as KPC-2 (n = 52), NDM-1 (n = 16), IMP-8 (n = 2), and VIM-1 (n = 1) producers. The isolates had been recovered mainly from urine, blood, and abdominal fluids among others, and some were from screening samples. After analyzing the virulence gene content, 76% of the isolates were considered ExPEC, although non-ExPEC isolates were also obtained from extraintestinal sites. Pan-genome phylogeny and clonal analysis showed great clonal diversity, although the first phylogroup in abundance was phylogroup A, harboring CC10 isolates, followed by phylogroup B2 with CC/ST131, mostly H30Rx, the subclone co-producing CTX-M-15. Phylogroups D, B1, C, F, and E were also detected with fewer strains. CC10 and CC/ST131 were found throughout the country. In addition, CC10 nucleated most metalloenzymes, such as NDM-1. Other relevant international clones were identified, such as CC/ST38, CC155, CC14/ST1193, and CC23. Two isolates co-produced KPC-2 and OXA-163 or OXA-439, a point mutation variant of OXA-163, and three isolates co-produced MCR-1 among other resistance genes. To conclude, in this work, we described the molecular epidemiology of carbapenemase-producing ExPEC in Argentina. Further studies are necessary to determine the plasmid families disseminating carbapenemases in ExPEC in this region. Frontiers Media S.A. 2022-03-18 /pmc/articles/PMC8971848/ /pubmed/35369469 http://dx.doi.org/10.3389/fmicb.2022.830209 Text en Copyright © 2022 Sanz, De Belder, de Mendieta, Faccone, Poklepovich, Lucero, Rapoport, Campos, Tuduri, Saavedra, Van der Ploeg, Rogé, Pasteran, Corso, Rosato and Gomez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sanz, María Belén
De Belder, Denise
de Mendieta, JM
Faccone, Diego
Poklepovich, Tomás
Lucero, Celeste
Rapoport, Melina
Campos, Josefina
Tuduri, Ezequiel
Saavedra, Mathew O.
Van der Ploeg, Claudia
Rogé, Ariel
Pasteran, Fernando
Corso, Alejandra
Rosato, Adriana E.
Gomez, Sonia A.
Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131
title Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131
title_full Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131
title_fullStr Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131
title_full_unstemmed Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131
title_short Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131
title_sort carbapenemase-producing extraintestinal pathogenic escherichia coli from argentina: clonal diversity and predominance of hyperepidemic clones cc10 and cc131
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971848/
https://www.ncbi.nlm.nih.gov/pubmed/35369469
http://dx.doi.org/10.3389/fmicb.2022.830209
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