Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live(TM) system. The setup we describe dissipates TTFields-related heat pro...

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Detalles Bibliográficos
Autores principales: Slangen, Paul L.G., Porat, Yaara, Mertz, Marjolijn, van den Broek, Bram, Jalink, Kees, de Gooijer, Mark C., van Tellingen, Olaf, Borst, Gerben R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971986/
https://www.ncbi.nlm.nih.gov/pubmed/35368806
http://dx.doi.org/10.1016/j.xpro.2022.101246
Descripción
Sumario:Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live(TM) system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields’ mechanism of action at the molecular level and facilitate the development of promising combination strategies.

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