Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live(TM) system. The setup we describe dissipates TTFields-related heat pro...

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Detalles Bibliográficos
Autores principales: Slangen, Paul L.G., Porat, Yaara, Mertz, Marjolijn, van den Broek, Bram, Jalink, Kees, de Gooijer, Mark C., van Tellingen, Olaf, Borst, Gerben R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971986/
https://www.ncbi.nlm.nih.gov/pubmed/35368806
http://dx.doi.org/10.1016/j.xpro.2022.101246
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author Slangen, Paul L.G.
Porat, Yaara
Mertz, Marjolijn
van den Broek, Bram
Jalink, Kees
de Gooijer, Mark C.
van Tellingen, Olaf
Borst, Gerben R.
author_facet Slangen, Paul L.G.
Porat, Yaara
Mertz, Marjolijn
van den Broek, Bram
Jalink, Kees
de Gooijer, Mark C.
van Tellingen, Olaf
Borst, Gerben R.
author_sort Slangen, Paul L.G.
collection PubMed
description Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live(TM) system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields’ mechanism of action at the molecular level and facilitate the development of promising combination strategies.
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spelling pubmed-89719862022-04-02 Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live Slangen, Paul L.G. Porat, Yaara Mertz, Marjolijn van den Broek, Bram Jalink, Kees de Gooijer, Mark C. van Tellingen, Olaf Borst, Gerben R. STAR Protoc Protocol Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live(TM) system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields’ mechanism of action at the molecular level and facilitate the development of promising combination strategies. Elsevier 2022-03-30 /pmc/articles/PMC8971986/ /pubmed/35368806 http://dx.doi.org/10.1016/j.xpro.2022.101246 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Slangen, Paul L.G.
Porat, Yaara
Mertz, Marjolijn
van den Broek, Bram
Jalink, Kees
de Gooijer, Mark C.
van Tellingen, Olaf
Borst, Gerben R.
Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live
title Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live
title_full Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live
title_fullStr Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live
title_full_unstemmed Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live
title_short Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live
title_sort protocol for live-cell imaging during tumor treating fields treatment with inovitro live
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971986/
https://www.ncbi.nlm.nih.gov/pubmed/35368806
http://dx.doi.org/10.1016/j.xpro.2022.101246
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