Cargando…
Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973405/ https://www.ncbi.nlm.nih.gov/pubmed/35032164 http://dx.doi.org/10.1093/jmicro/dfac002 |
_version_ | 1784680035625467904 |
---|---|
author | Kohno, Yuji Nakamura, Akiho Morishita, Shigeyuki Shibata, Naoya |
author_facet | Kohno, Yuji Nakamura, Akiho Morishita, Shigeyuki Shibata, Naoya |
author_sort | Kohno, Yuji |
collection | PubMed |
description | Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample bends or thickness changes. These additional contrasts are called diffraction contrasts, and sometimes make it difficult to extract pure electromagnetic field information from the experimental DPC images. In this study, we developed a beam scan system that can acquire many DPC images from the same sample region with arbitrarily varying incident beam tilt angles to the sample. Then, these images are precisely averaged to form tilt-scan averaged DPC images. It is shown that the diffraction contrast can be effectively reduced in the tilt-scan averaged DPC images. |
format | Online Article Text |
id | pubmed-8973405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89734052022-04-04 Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy Kohno, Yuji Nakamura, Akiho Morishita, Shigeyuki Shibata, Naoya Microscopy (Oxf) Article Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample bends or thickness changes. These additional contrasts are called diffraction contrasts, and sometimes make it difficult to extract pure electromagnetic field information from the experimental DPC images. In this study, we developed a beam scan system that can acquire many DPC images from the same sample region with arbitrarily varying incident beam tilt angles to the sample. Then, these images are precisely averaged to form tilt-scan averaged DPC images. It is shown that the diffraction contrast can be effectively reduced in the tilt-scan averaged DPC images. Oxford University Press 2022-01-15 /pmc/articles/PMC8973405/ /pubmed/35032164 http://dx.doi.org/10.1093/jmicro/dfac002 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Kohno, Yuji Nakamura, Akiho Morishita, Shigeyuki Shibata, Naoya Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
title | Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
title_full | Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
title_fullStr | Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
title_full_unstemmed | Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
title_short | Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
title_sort | development of tilt-scan system for differential phase contrast scanning transmission electron microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973405/ https://www.ncbi.nlm.nih.gov/pubmed/35032164 http://dx.doi.org/10.1093/jmicro/dfac002 |
work_keys_str_mv | AT kohnoyuji developmentoftiltscansystemfordifferentialphasecontrastscanningtransmissionelectronmicroscopy AT nakamuraakiho developmentoftiltscansystemfordifferentialphasecontrastscanningtransmissionelectronmicroscopy AT morishitashigeyuki developmentoftiltscansystemfordifferentialphasecontrastscanningtransmissionelectronmicroscopy AT shibatanaoya developmentoftiltscansystemfordifferentialphasecontrastscanningtransmissionelectronmicroscopy |