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Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy

Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample...

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Detalles Bibliográficos
Autores principales: Kohno, Yuji, Nakamura, Akiho, Morishita, Shigeyuki, Shibata, Naoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973405/
https://www.ncbi.nlm.nih.gov/pubmed/35032164
http://dx.doi.org/10.1093/jmicro/dfac002
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author Kohno, Yuji
Nakamura, Akiho
Morishita, Shigeyuki
Shibata, Naoya
author_facet Kohno, Yuji
Nakamura, Akiho
Morishita, Shigeyuki
Shibata, Naoya
author_sort Kohno, Yuji
collection PubMed
description Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample bends or thickness changes. These additional contrasts are called diffraction contrasts, and sometimes make it difficult to extract pure electromagnetic field information from the experimental DPC images. In this study, we developed a beam scan system that can acquire many DPC images from the same sample region with arbitrarily varying incident beam tilt angles to the sample. Then, these images are precisely averaged to form tilt-scan averaged DPC images. It is shown that the diffraction contrast can be effectively reduced in the tilt-scan averaged DPC images.
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spelling pubmed-89734052022-04-04 Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy Kohno, Yuji Nakamura, Akiho Morishita, Shigeyuki Shibata, Naoya Microscopy (Oxf) Article Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample bends or thickness changes. These additional contrasts are called diffraction contrasts, and sometimes make it difficult to extract pure electromagnetic field information from the experimental DPC images. In this study, we developed a beam scan system that can acquire many DPC images from the same sample region with arbitrarily varying incident beam tilt angles to the sample. Then, these images are precisely averaged to form tilt-scan averaged DPC images. It is shown that the diffraction contrast can be effectively reduced in the tilt-scan averaged DPC images. Oxford University Press 2022-01-15 /pmc/articles/PMC8973405/ /pubmed/35032164 http://dx.doi.org/10.1093/jmicro/dfac002 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kohno, Yuji
Nakamura, Akiho
Morishita, Shigeyuki
Shibata, Naoya
Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
title Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
title_full Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
title_fullStr Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
title_full_unstemmed Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
title_short Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
title_sort development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973405/
https://www.ncbi.nlm.nih.gov/pubmed/35032164
http://dx.doi.org/10.1093/jmicro/dfac002
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