Cargando…

Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum

BACKGROUND: The rhizome of Polygonatum kingianum Coll. et Hemsl (P. kingianum) is a crucial traditional Chinese medicine, but severe bud dormancy occurs during early rhizome development. Low temperature is a positive factor affecting dormancy release, whereas the variation in carbohydrates during do...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yue, Liu, Tao, Ma, Changjian, Li, Guoqing, Wang, Xinhong, Wang, Jianghui, Chang, Jin, Guan, Cong, Yao, Huimin, Dong, Xuehui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973533/
https://www.ncbi.nlm.nih.gov/pubmed/35365083
http://dx.doi.org/10.1186/s12870-022-03558-0
_version_ 1784680058245349376
author Wang, Yue
Liu, Tao
Ma, Changjian
Li, Guoqing
Wang, Xinhong
Wang, Jianghui
Chang, Jin
Guan, Cong
Yao, Huimin
Dong, Xuehui
author_facet Wang, Yue
Liu, Tao
Ma, Changjian
Li, Guoqing
Wang, Xinhong
Wang, Jianghui
Chang, Jin
Guan, Cong
Yao, Huimin
Dong, Xuehui
author_sort Wang, Yue
collection PubMed
description BACKGROUND: The rhizome of Polygonatum kingianum Coll. et Hemsl (P. kingianum) is a crucial traditional Chinese medicine, but severe bud dormancy occurs during early rhizome development. Low temperature is a positive factor affecting dormancy release, whereas the variation in carbohydrates during dormancy release has not been investigated systematically. Therefore, the sugar content, related metabolic pathways and gene co-expression were analysed to elucidate the regulatory mechanism of carbohydrates during dormancy release in the P. kingianum rhizome bud. RESULTS: During dormancy transition, starch and sucrose (Suc) exhibited opposing trends in the P. kingianum rhizome bud, representing a critical indicator of dormancy release. Galactose (Gal) and raffinose (Raf) were increased in content and synthesis. Glucose (Glc), cellulose (Cel), mannose (Man), arabinose (Ara), rhamnose (Rha) and stachyose (Sta) showed various changes, indicating their different roles in breaking rhizome bud dormancy in P. kingianum. At the beginning of dormancy release, Glc metabolism may be dominated by anaerobic oxidation (glycolysis followed by ethanol fermentation). After entering the S3 stage, the tricarboxylic acid cycle (TCA) and pentose phosphate pathway (PPP) were may be more active possibly. In the gene co-expression network comprising carbohydrates and hormones, HYD1 was identified as a hub gene, and numerous interactions centred on STS/SUS were also observed, suggesting the essential role of brassinosteroids (BRs), Raf and Suc in the regulatory network. CONCLUSION: We revealed cold-responsive genes related to carbohydrate metabolism, suggesting regulatory mechanisms of sugar during dormancy release in the P. kingianum rhizome bud. Additionally, gene co-expression analysis revealed possible interactions between sugar and hormone signalling, providing new insight into the dormancy release mechanism in P. kingianum rhizome buds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03558-0.
format Online
Article
Text
id pubmed-8973533
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-89735332022-04-02 Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum Wang, Yue Liu, Tao Ma, Changjian Li, Guoqing Wang, Xinhong Wang, Jianghui Chang, Jin Guan, Cong Yao, Huimin Dong, Xuehui BMC Plant Biol Research BACKGROUND: The rhizome of Polygonatum kingianum Coll. et Hemsl (P. kingianum) is a crucial traditional Chinese medicine, but severe bud dormancy occurs during early rhizome development. Low temperature is a positive factor affecting dormancy release, whereas the variation in carbohydrates during dormancy release has not been investigated systematically. Therefore, the sugar content, related metabolic pathways and gene co-expression were analysed to elucidate the regulatory mechanism of carbohydrates during dormancy release in the P. kingianum rhizome bud. RESULTS: During dormancy transition, starch and sucrose (Suc) exhibited opposing trends in the P. kingianum rhizome bud, representing a critical indicator of dormancy release. Galactose (Gal) and raffinose (Raf) were increased in content and synthesis. Glucose (Glc), cellulose (Cel), mannose (Man), arabinose (Ara), rhamnose (Rha) and stachyose (Sta) showed various changes, indicating their different roles in breaking rhizome bud dormancy in P. kingianum. At the beginning of dormancy release, Glc metabolism may be dominated by anaerobic oxidation (glycolysis followed by ethanol fermentation). After entering the S3 stage, the tricarboxylic acid cycle (TCA) and pentose phosphate pathway (PPP) were may be more active possibly. In the gene co-expression network comprising carbohydrates and hormones, HYD1 was identified as a hub gene, and numerous interactions centred on STS/SUS were also observed, suggesting the essential role of brassinosteroids (BRs), Raf and Suc in the regulatory network. CONCLUSION: We revealed cold-responsive genes related to carbohydrate metabolism, suggesting regulatory mechanisms of sugar during dormancy release in the P. kingianum rhizome bud. Additionally, gene co-expression analysis revealed possible interactions between sugar and hormone signalling, providing new insight into the dormancy release mechanism in P. kingianum rhizome buds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03558-0. BioMed Central 2022-04-01 /pmc/articles/PMC8973533/ /pubmed/35365083 http://dx.doi.org/10.1186/s12870-022-03558-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Yue
Liu, Tao
Ma, Changjian
Li, Guoqing
Wang, Xinhong
Wang, Jianghui
Chang, Jin
Guan, Cong
Yao, Huimin
Dong, Xuehui
Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum
title Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum
title_full Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum
title_fullStr Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum
title_full_unstemmed Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum
title_short Carbohydrate regulation response to cold during rhizome bud dormancy release in Polygonatum kingianum
title_sort carbohydrate regulation response to cold during rhizome bud dormancy release in polygonatum kingianum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973533/
https://www.ncbi.nlm.nih.gov/pubmed/35365083
http://dx.doi.org/10.1186/s12870-022-03558-0
work_keys_str_mv AT wangyue carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT liutao carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT machangjian carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT liguoqing carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT wangxinhong carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT wangjianghui carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT changjin carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT guancong carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT yaohuimin carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum
AT dongxuehui carbohydrateregulationresponsetocoldduringrhizomebuddormancyreleaseinpolygonatumkingianum