Cargando…
LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p
Our previous studies indicate that long noncoding RNA (lncRNA) LINC00467 can act as an oncogene to participate in the malignant progression of glioma, but the underlying molecular mechanism remains to be studied further. This study aimed to explore the biological role of the LINC00467/miR-339-3p/ in...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973818/ https://www.ncbi.nlm.nih.gov/pubmed/35156508 http://dx.doi.org/10.1080/21655979.2021.2018098 |
_version_ | 1784680124612870144 |
---|---|
author | Zhang, Yin Zhang, Yaxuan Wang, Sen Cao, Boqiang Hu, Daling Jia, Junli Wang, Yuhang Chen, Luyao Li, Jiaming Tang, Huamin Liu, Hongyi |
author_facet | Zhang, Yin Zhang, Yaxuan Wang, Sen Cao, Boqiang Hu, Daling Jia, Junli Wang, Yuhang Chen, Luyao Li, Jiaming Tang, Huamin Liu, Hongyi |
author_sort | Zhang, Yin |
collection | PubMed |
description | Our previous studies indicate that long noncoding RNA (lncRNA) LINC00467 can act as an oncogene to participate in the malignant progression of glioma, but the underlying molecular mechanism remains to be studied further. This study aimed to explore the biological role of the LINC00467/miR-339-3p/ inositol hexakisphosphate kinase 2 (IP6K2) regulatory axis in glioma. The Cancer Genome Atlas (TCGA), Oncomine databases and reverse transcription‑quantitative PCR (RT‑qPCR) were used to analyze IP6K2 expression in glioma. RT-PCR, EdU and transwell assays were conducted to observe the effect of IP6K2 on glioma cell proliferation, migration and invasion. Using bioinformatics analysis, RT-PCR, and dual luciferase reporter gene assay, the potential role of the LINC00467/miR-339-3p/IP6K2 regulatory axis in glioma was verified. The results showed that IP6K2 was up-regulated in glioma tissues and cell lines. Moreover, the expression level of IP6K2 was correlated with the clinical features of glioma patients. In vitro and in vivo experiments indicated that IP6K2 overexpression could promote the proliferation, migration, and invasion of glioma cells. Further bioinformatics analysis and in vitro assays revealed that LINC00467 could promote IP6K2 expression by binding to miR-339-3p and promote the malignant progression of glioma. Overall, LINC00467 could upregulate IP6K2 by binding to miR-339-3p and promote the proliferation, migration, and invasion of glioma cells. The LINC00467/miR-339-3p/IP6K2 regulatory axis might be a potential therapeutic target for glioma. |
format | Online Article Text |
id | pubmed-8973818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-89738182022-04-02 LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p Zhang, Yin Zhang, Yaxuan Wang, Sen Cao, Boqiang Hu, Daling Jia, Junli Wang, Yuhang Chen, Luyao Li, Jiaming Tang, Huamin Liu, Hongyi Bioengineered Research Paper Our previous studies indicate that long noncoding RNA (lncRNA) LINC00467 can act as an oncogene to participate in the malignant progression of glioma, but the underlying molecular mechanism remains to be studied further. This study aimed to explore the biological role of the LINC00467/miR-339-3p/ inositol hexakisphosphate kinase 2 (IP6K2) regulatory axis in glioma. The Cancer Genome Atlas (TCGA), Oncomine databases and reverse transcription‑quantitative PCR (RT‑qPCR) were used to analyze IP6K2 expression in glioma. RT-PCR, EdU and transwell assays were conducted to observe the effect of IP6K2 on glioma cell proliferation, migration and invasion. Using bioinformatics analysis, RT-PCR, and dual luciferase reporter gene assay, the potential role of the LINC00467/miR-339-3p/IP6K2 regulatory axis in glioma was verified. The results showed that IP6K2 was up-regulated in glioma tissues and cell lines. Moreover, the expression level of IP6K2 was correlated with the clinical features of glioma patients. In vitro and in vivo experiments indicated that IP6K2 overexpression could promote the proliferation, migration, and invasion of glioma cells. Further bioinformatics analysis and in vitro assays revealed that LINC00467 could promote IP6K2 expression by binding to miR-339-3p and promote the malignant progression of glioma. Overall, LINC00467 could upregulate IP6K2 by binding to miR-339-3p and promote the proliferation, migration, and invasion of glioma cells. The LINC00467/miR-339-3p/IP6K2 regulatory axis might be a potential therapeutic target for glioma. Taylor & Francis 2022-02-13 /pmc/articles/PMC8973818/ /pubmed/35156508 http://dx.doi.org/10.1080/21655979.2021.2018098 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Zhang, Yin Zhang, Yaxuan Wang, Sen Cao, Boqiang Hu, Daling Jia, Junli Wang, Yuhang Chen, Luyao Li, Jiaming Tang, Huamin Liu, Hongyi LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p |
title | LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p |
title_full | LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p |
title_fullStr | LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p |
title_full_unstemmed | LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p |
title_short | LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p |
title_sort | linc00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to mir-339-3p |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973818/ https://www.ncbi.nlm.nih.gov/pubmed/35156508 http://dx.doi.org/10.1080/21655979.2021.2018098 |
work_keys_str_mv | AT zhangyin linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT zhangyaxuan linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT wangsen linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT caoboqiang linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT hudaling linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT jiajunli linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT wangyuhang linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT chenluyao linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT lijiaming linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT tanghuamin linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p AT liuhongyi linc00467facilitatestheproliferationmigrationandinvasionofgliomaviapromotingtheexpressionofinositolhexakisphosphatekinase2bybindingtomir3393p |