NDF is a transcription factor that stimulates elongation by RNA polymerase II

RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF bind...

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Detalles Bibliográficos
Autores principales: Fei, Jia, Xu, Jun, Li, Ziwei, Xu, Kevin, Wang, Dong, Kassavetis, George A., Kadonaga, James T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973848/
https://www.ncbi.nlm.nih.gov/pubmed/35273076
http://dx.doi.org/10.1101/gad.349150.121
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author Fei, Jia
Xu, Jun
Li, Ziwei
Xu, Kevin
Wang, Dong
Kassavetis, George A.
Kadonaga, James T.
author_facet Fei, Jia
Xu, Jun
Li, Ziwei
Xu, Kevin
Wang, Dong
Kassavetis, George A.
Kadonaga, James T.
author_sort Fei, Jia
collection PubMed
description RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF binds directly to Pol II and enhances elongation by a different mechanism than that used by transcription factor TFIIS. Moreover, yeast Pdp3, which is related to NDF, binds to Pol II and stimulates elongation. Thus, NDF is a Pol II binding transcription elongation factor that is localized over gene bodies and is conserved from yeast to humans.
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spelling pubmed-89738482022-09-01 NDF is a transcription factor that stimulates elongation by RNA polymerase II Fei, Jia Xu, Jun Li, Ziwei Xu, Kevin Wang, Dong Kassavetis, George A. Kadonaga, James T. Genes Dev Research Communication RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF binds directly to Pol II and enhances elongation by a different mechanism than that used by transcription factor TFIIS. Moreover, yeast Pdp3, which is related to NDF, binds to Pol II and stimulates elongation. Thus, NDF is a Pol II binding transcription elongation factor that is localized over gene bodies and is conserved from yeast to humans. Cold Spring Harbor Laboratory Press 2022-03-01 /pmc/articles/PMC8973848/ /pubmed/35273076 http://dx.doi.org/10.1101/gad.349150.121 Text en © 2022 Fei et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by-nc/4.0/This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Research Communication
Fei, Jia
Xu, Jun
Li, Ziwei
Xu, Kevin
Wang, Dong
Kassavetis, George A.
Kadonaga, James T.
NDF is a transcription factor that stimulates elongation by RNA polymerase II
title NDF is a transcription factor that stimulates elongation by RNA polymerase II
title_full NDF is a transcription factor that stimulates elongation by RNA polymerase II
title_fullStr NDF is a transcription factor that stimulates elongation by RNA polymerase II
title_full_unstemmed NDF is a transcription factor that stimulates elongation by RNA polymerase II
title_short NDF is a transcription factor that stimulates elongation by RNA polymerase II
title_sort ndf is a transcription factor that stimulates elongation by rna polymerase ii
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973848/
https://www.ncbi.nlm.nih.gov/pubmed/35273076
http://dx.doi.org/10.1101/gad.349150.121
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