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Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection

Intrauterine infection is linked to adverse pregnancy outcomes in pregnant women. Neonates from parturients with intrauterine infection are usually treated with antibiotics, but their gut microbiota and metabolome are seldom studied. In this study, we collected fecal samples from antibiotic-treated...

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Autores principales: Li, Huitao, Fu, Lei, Chen, Xueyu, Xu, Heng, Jing, Qinlong, Yang, Chuanzhong, Wan, Zhengwei, Chen, Yiran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974630/
https://www.ncbi.nlm.nih.gov/pubmed/35372104
http://dx.doi.org/10.3389/fcimb.2022.817832
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author Li, Huitao
Fu, Lei
Chen, Xueyu
Xu, Heng
Jing, Qinlong
Yang, Chuanzhong
Wan, Zhengwei
Chen, Yiran
author_facet Li, Huitao
Fu, Lei
Chen, Xueyu
Xu, Heng
Jing, Qinlong
Yang, Chuanzhong
Wan, Zhengwei
Chen, Yiran
author_sort Li, Huitao
collection PubMed
description Intrauterine infection is linked to adverse pregnancy outcomes in pregnant women. Neonates from parturients with intrauterine infection are usually treated with antibiotics, but their gut microbiota and metabolome are seldom studied. In this study, we collected fecal samples from antibiotic-treated neonates of parturients with intrauterine infection (intrauterine infection group), parturients with non-intrauterine infection (antibiotic group), and untreated neonates of healthy parturients (control group). 16S rRNA gene sequencing and untargeted metabolomics analyses were performed. Our results revealed that the α-diversity of intrauterine infection group differed from that of control group. There were significant differences in β-diversity between intrauterine infection group and control group, between antibiotic group and the control group, but there was no difference between the intrauterine infection and antibiotic groups, implying that antibiotic use has an obvious effect on β-diversity and that the effects of intrauterine infection on β-diversity cannot be identified. Enterococcus was more abundant in intrauterine infection and antibiotic groups than in control group. Gut metabolite differences in intrauterine infection group and antibiotic group (only in negative ion mode) from control group were observed, but no difference between intrauterine infection group and antibiotic group was observed. N-formyl-L-methionine was the most discriminant metabolite between intrauterine infection group and control group. Primary and secondary bile acid biosynthesis, bile secretion, and cholesterol metabolism pathways were altered, and the abundances of bile acids and bile salts were altered in intrauterine infection group compared with control group. Alterations in cholesterol metabolism, arginine biosynthesis and bile secretion pathways were observed both in intrauterine infection and antibiotic groups, which might be caused by the use of antibiotics. In conclusion, we provided a preliminary description of the gut microbiota and gut metabolites in antibiotics-treated neonates from intrauterine infection parturients. Our findings did not show intrauterine infection has a separate role in neonatal gut microbiota dysbiosis, while supporting the idea that antibiotics should be used with caution during neonatal therapy.
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spelling pubmed-89746302022-04-02 Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection Li, Huitao Fu, Lei Chen, Xueyu Xu, Heng Jing, Qinlong Yang, Chuanzhong Wan, Zhengwei Chen, Yiran Front Cell Infect Microbiol Cellular and Infection Microbiology Intrauterine infection is linked to adverse pregnancy outcomes in pregnant women. Neonates from parturients with intrauterine infection are usually treated with antibiotics, but their gut microbiota and metabolome are seldom studied. In this study, we collected fecal samples from antibiotic-treated neonates of parturients with intrauterine infection (intrauterine infection group), parturients with non-intrauterine infection (antibiotic group), and untreated neonates of healthy parturients (control group). 16S rRNA gene sequencing and untargeted metabolomics analyses were performed. Our results revealed that the α-diversity of intrauterine infection group differed from that of control group. There were significant differences in β-diversity between intrauterine infection group and control group, between antibiotic group and the control group, but there was no difference between the intrauterine infection and antibiotic groups, implying that antibiotic use has an obvious effect on β-diversity and that the effects of intrauterine infection on β-diversity cannot be identified. Enterococcus was more abundant in intrauterine infection and antibiotic groups than in control group. Gut metabolite differences in intrauterine infection group and antibiotic group (only in negative ion mode) from control group were observed, but no difference between intrauterine infection group and antibiotic group was observed. N-formyl-L-methionine was the most discriminant metabolite between intrauterine infection group and control group. Primary and secondary bile acid biosynthesis, bile secretion, and cholesterol metabolism pathways were altered, and the abundances of bile acids and bile salts were altered in intrauterine infection group compared with control group. Alterations in cholesterol metabolism, arginine biosynthesis and bile secretion pathways were observed both in intrauterine infection and antibiotic groups, which might be caused by the use of antibiotics. In conclusion, we provided a preliminary description of the gut microbiota and gut metabolites in antibiotics-treated neonates from intrauterine infection parturients. Our findings did not show intrauterine infection has a separate role in neonatal gut microbiota dysbiosis, while supporting the idea that antibiotics should be used with caution during neonatal therapy. Frontiers Media S.A. 2022-03-18 /pmc/articles/PMC8974630/ /pubmed/35372104 http://dx.doi.org/10.3389/fcimb.2022.817832 Text en Copyright © 2022 Li, Fu, Chen, Xu, Jing, Yang, Wan and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Huitao
Fu, Lei
Chen, Xueyu
Xu, Heng
Jing, Qinlong
Yang, Chuanzhong
Wan, Zhengwei
Chen, Yiran
Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection
title Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection
title_full Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection
title_fullStr Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection
title_full_unstemmed Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection
title_short Gut Microbiota and Metabolome Description of Antibiotic-Treated Neonates From Parturients With Intrauterine Infection
title_sort gut microbiota and metabolome description of antibiotic-treated neonates from parturients with intrauterine infection
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974630/
https://www.ncbi.nlm.nih.gov/pubmed/35372104
http://dx.doi.org/10.3389/fcimb.2022.817832
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