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Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas
Background: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974670/ https://www.ncbi.nlm.nih.gov/pubmed/35386988 http://dx.doi.org/10.3389/falgy.2021.691627 |
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author | Wangorsch, Andrea Jamin, Annette Eichhorn, Stephanie Pablos, Isabel Sharma, Swati Schweidler, Bettina Kastner, Bianca Wildner, Sabrina Saloga, Joachim Führer, Frank Reyna Orozco, Reinaldo Rafael Sherkat, Roya Sadeghi, Somayeh Teifoori, Fardis Park, Jung-Won Briza, Peter Vieths, Stefan Ferreira, Fatima Arora, Naveen Lidholm, Jonas Gadermaier, Gabriele Scheurer, Stephan |
author_facet | Wangorsch, Andrea Jamin, Annette Eichhorn, Stephanie Pablos, Isabel Sharma, Swati Schweidler, Bettina Kastner, Bianca Wildner, Sabrina Saloga, Joachim Führer, Frank Reyna Orozco, Reinaldo Rafael Sherkat, Roya Sadeghi, Somayeh Teifoori, Fardis Park, Jung-Won Briza, Peter Vieths, Stefan Ferreira, Fatima Arora, Naveen Lidholm, Jonas Gadermaier, Gabriele Scheurer, Stephan |
author_sort | Wangorsch, Andrea |
collection | PubMed |
description | Background: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD). Objective: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy. Methods: A panel of nine recombinant Periplaneta allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients (n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach (P. americana and/or Blattella germanica), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing. Results: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients (r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta-derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered. Conclusion: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens. |
format | Online Article Text |
id | pubmed-8974670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89746702022-04-05 Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas Wangorsch, Andrea Jamin, Annette Eichhorn, Stephanie Pablos, Isabel Sharma, Swati Schweidler, Bettina Kastner, Bianca Wildner, Sabrina Saloga, Joachim Führer, Frank Reyna Orozco, Reinaldo Rafael Sherkat, Roya Sadeghi, Somayeh Teifoori, Fardis Park, Jung-Won Briza, Peter Vieths, Stefan Ferreira, Fatima Arora, Naveen Lidholm, Jonas Gadermaier, Gabriele Scheurer, Stephan Front Allergy Allergy Background: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD). Objective: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy. Methods: A panel of nine recombinant Periplaneta allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients (n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach (P. americana and/or Blattella germanica), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing. Results: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients (r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta-derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered. Conclusion: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens. Frontiers Media S.A. 2021-06-29 /pmc/articles/PMC8974670/ /pubmed/35386988 http://dx.doi.org/10.3389/falgy.2021.691627 Text en Copyright © 2021 Wangorsch, Jamin, Eichhorn, Pablos, Sharma, Schweidler, Kastner, Wildner, Saloga, Führer, Reyna Orozco, Sherkat, Sadeghi, Teifoori, Park, Briza, Vieths, Ferreira, Arora, Lidholm, Gadermaier and Scheurer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Allergy Wangorsch, Andrea Jamin, Annette Eichhorn, Stephanie Pablos, Isabel Sharma, Swati Schweidler, Bettina Kastner, Bianca Wildner, Sabrina Saloga, Joachim Führer, Frank Reyna Orozco, Reinaldo Rafael Sherkat, Roya Sadeghi, Somayeh Teifoori, Fardis Park, Jung-Won Briza, Peter Vieths, Stefan Ferreira, Fatima Arora, Naveen Lidholm, Jonas Gadermaier, Gabriele Scheurer, Stephan Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
title | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
title_full | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
title_fullStr | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
title_full_unstemmed | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
title_short | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
title_sort | component-resolved diagnosis of american cockroach (periplaneta americana) allergy in patients from different geographical areas |
topic | Allergy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974670/ https://www.ncbi.nlm.nih.gov/pubmed/35386988 http://dx.doi.org/10.3389/falgy.2021.691627 |
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