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The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network
Although the nose, as a gateway for organism–environment interactions, may have a key role in asthmatic exacerbation, the rhinobiome of exacerbated children with asthma was widely neglected to date. The aim of this study is to understand the microbiome, the microbial immunology, and the proteome of...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974812/ https://www.ncbi.nlm.nih.gov/pubmed/35386977 http://dx.doi.org/10.3389/falgy.2021.667562 |
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author | Aydin, Malik Weisser, Cornelius Rué, Olivier Mariadassou, Mahendra Maaß, Sandra Behrendt, Ann-Kathrin Jaszczyszyn, Yan Heilker, Tatje Spaeth, Maximilian Vogel, Silvia Lutz, Sören Ahmad-Nejad, Parviz Graf, Viktoria Bellm, Aliyah Weisser, Christoph Naumova, Ella A. Arnold, Wolfgang H. Ehrhardt, Anja Meyer-Bahlburg, Almut Becher, Dörte Postberg, Jan Ghebremedhin, Beniam Wirth, Stefan |
author_facet | Aydin, Malik Weisser, Cornelius Rué, Olivier Mariadassou, Mahendra Maaß, Sandra Behrendt, Ann-Kathrin Jaszczyszyn, Yan Heilker, Tatje Spaeth, Maximilian Vogel, Silvia Lutz, Sören Ahmad-Nejad, Parviz Graf, Viktoria Bellm, Aliyah Weisser, Christoph Naumova, Ella A. Arnold, Wolfgang H. Ehrhardt, Anja Meyer-Bahlburg, Almut Becher, Dörte Postberg, Jan Ghebremedhin, Beniam Wirth, Stefan |
author_sort | Aydin, Malik |
collection | PubMed |
description | Although the nose, as a gateway for organism–environment interactions, may have a key role in asthmatic exacerbation, the rhinobiome of exacerbated children with asthma was widely neglected to date. The aim of this study is to understand the microbiome, the microbial immunology, and the proteome of exacerbated children and adolescents with wheeze and asthma. Considering that a certain proportion of wheezers may show a progression to asthma, the comparison of both groups provides important information regarding clinical and phenotype stratification. Thus, deep nasopharyngeal swab specimens, nasal epithelial spheroid (NAEsp) cultures, and blood samples of acute exacerbated wheezers (WH), asthmatics (AB), and healthy controls (HC) were used for culture (n = 146), 16 S-rRNA gene amplicon sequencing (n = 64), and proteomic and cytokine analyses. Interestingly, Proteobacteria were over-represented in WH, whereas Firmicutes and Bacteroidetes were associated with AB. In contrast, Actinobacteria commonly colonized HCs. Moreover, Staphylococcaceae, Enterobacteriaceae, Burkholderiaceae, Xanthobacteraceae, and Sphingomonadaceae were significantly more abundant in AB compared to WH and HC. The α-diversity analyses demonstrated an increase of bacterial abundance levels in atopic AB and a decrease in WH samples. Microbiome profiles of atopic WH differed significantly from atopic AB, whereby atopic samples of WH were more homogeneous than those of non-atopic subjects. The NAEsp bacterial exposure experiments provided a disrupted epithelial cell integrity, a cytokine release, and cohort-specific proteomic differences especially for Moraxella catarrhalis cultures. This comprehensive dataset contributes to a deeper insight into the poorly understood plasticity of the nasal microbiota, and, in particular, may enforce our understanding in the pathogenesis of asthma exacerbation in childhood. |
format | Online Article Text |
id | pubmed-8974812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89748122022-04-05 The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network Aydin, Malik Weisser, Cornelius Rué, Olivier Mariadassou, Mahendra Maaß, Sandra Behrendt, Ann-Kathrin Jaszczyszyn, Yan Heilker, Tatje Spaeth, Maximilian Vogel, Silvia Lutz, Sören Ahmad-Nejad, Parviz Graf, Viktoria Bellm, Aliyah Weisser, Christoph Naumova, Ella A. Arnold, Wolfgang H. Ehrhardt, Anja Meyer-Bahlburg, Almut Becher, Dörte Postberg, Jan Ghebremedhin, Beniam Wirth, Stefan Front Allergy Allergy Although the nose, as a gateway for organism–environment interactions, may have a key role in asthmatic exacerbation, the rhinobiome of exacerbated children with asthma was widely neglected to date. The aim of this study is to understand the microbiome, the microbial immunology, and the proteome of exacerbated children and adolescents with wheeze and asthma. Considering that a certain proportion of wheezers may show a progression to asthma, the comparison of both groups provides important information regarding clinical and phenotype stratification. Thus, deep nasopharyngeal swab specimens, nasal epithelial spheroid (NAEsp) cultures, and blood samples of acute exacerbated wheezers (WH), asthmatics (AB), and healthy controls (HC) were used for culture (n = 146), 16 S-rRNA gene amplicon sequencing (n = 64), and proteomic and cytokine analyses. Interestingly, Proteobacteria were over-represented in WH, whereas Firmicutes and Bacteroidetes were associated with AB. In contrast, Actinobacteria commonly colonized HCs. Moreover, Staphylococcaceae, Enterobacteriaceae, Burkholderiaceae, Xanthobacteraceae, and Sphingomonadaceae were significantly more abundant in AB compared to WH and HC. The α-diversity analyses demonstrated an increase of bacterial abundance levels in atopic AB and a decrease in WH samples. Microbiome profiles of atopic WH differed significantly from atopic AB, whereby atopic samples of WH were more homogeneous than those of non-atopic subjects. The NAEsp bacterial exposure experiments provided a disrupted epithelial cell integrity, a cytokine release, and cohort-specific proteomic differences especially for Moraxella catarrhalis cultures. This comprehensive dataset contributes to a deeper insight into the poorly understood plasticity of the nasal microbiota, and, in particular, may enforce our understanding in the pathogenesis of asthma exacerbation in childhood. Frontiers Media S.A. 2021-05-31 /pmc/articles/PMC8974812/ /pubmed/35386977 http://dx.doi.org/10.3389/falgy.2021.667562 Text en Copyright © 2021 Aydin, Weisser, Rué, Mariadassou, Maaß, Behrendt, Jaszczyszyn, Heilker, Spaeth, Vogel, Lutz, Ahmad-Nejad, Graf, Bellm, Weisser, Naumova, Arnold, Ehrhardt, Meyer-Bahlburg, Becher, Postberg, Ghebremedhin and Wirth. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Allergy Aydin, Malik Weisser, Cornelius Rué, Olivier Mariadassou, Mahendra Maaß, Sandra Behrendt, Ann-Kathrin Jaszczyszyn, Yan Heilker, Tatje Spaeth, Maximilian Vogel, Silvia Lutz, Sören Ahmad-Nejad, Parviz Graf, Viktoria Bellm, Aliyah Weisser, Christoph Naumova, Ella A. Arnold, Wolfgang H. Ehrhardt, Anja Meyer-Bahlburg, Almut Becher, Dörte Postberg, Jan Ghebremedhin, Beniam Wirth, Stefan The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network |
title | The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network |
title_full | The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network |
title_fullStr | The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network |
title_full_unstemmed | The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network |
title_short | The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network |
title_sort | rhinobiome of exacerbated wheezers and asthmatics: insights from a german pediatric exacerbation network |
topic | Allergy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974812/ https://www.ncbi.nlm.nih.gov/pubmed/35386977 http://dx.doi.org/10.3389/falgy.2021.667562 |
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