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Successful ex vivo expansion of mouse hematopoietic stem cells

Ex vivo expansion of hematopoietic stem cells (HSCs) is considered the holy grail in stem cell biology and therapy, as it has long been difficult to make this procedure possible. Yamazaki's research team has established new, polyvinyl alcohol-based culture conditions and shown a significant exp...

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Detalles Bibliográficos
Autor principal: Ema, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974897/
https://www.ncbi.nlm.nih.gov/pubmed/35402802
http://dx.doi.org/10.1097/BS9.0000000000000006
Descripción
Sumario:Ex vivo expansion of hematopoietic stem cells (HSCs) is considered the holy grail in stem cell biology and therapy, as it has long been difficult to make this procedure possible. Yamazaki's research team has established new, polyvinyl alcohol-based culture conditions and shown a significant expansion of mouse HSCs from a small number of cells after a month of culture. Surprisingly, expanded HSCs were able to reconstitute unconditioned normal mice. There is generally a technical concern in limiting dilution assay to estimate a fold-expansion of HSCs. But, this work paves the way toward expansion of human HSCs useful for transplantation medicine.