When blood development meets single-cell transcriptomics
Blood cells arise during embryonic development by three temporally distinct waves. Belonging to the third wave, hematopoietic stem cells (HSCs) are generated from hemogenic endothelium via endothelial-to-hematopoietic transition in mid-gestational embryos. Recently, studies combined with single-cell...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974905/ https://www.ncbi.nlm.nih.gov/pubmed/35402794 http://dx.doi.org/10.1097/BS9.0000000000000007 |
Sumario: | Blood cells arise during embryonic development by three temporally distinct waves. Belonging to the third wave, hematopoietic stem cells (HSCs) are generated from hemogenic endothelium via endothelial-to-hematopoietic transition in mid-gestational embryos. Recently, studies combined with single-cell transcriptomics have provided massive new insights into the molecular evolutions and the underlying mechanisms of distinct waves of hematopoietic specification. In this review, we discuss the current single-cell profiling techniques, the most recent novel findings involved in the generation of distinct waves of blood cells, especially the HSCs, using single-cell transcriptional profiling combined with functional evaluations, and the perspectives to use the accumulating huge single-cell transcriptional data sets to study developmental hematopoiesis. |
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