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The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum

Many bacterial species, including several pathogens, can enter a so-called “viable but non-culturable” (VBNC) state when subjected to stress. Bacteria in the VBNC state are metabolically active but have lost their ability to grow on standard culture media, which compromises their detection by conven...

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Autores principales: Lotoux, Aurélie, Milohanic, Eliane, Bierne, Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974916/
https://www.ncbi.nlm.nih.gov/pubmed/35372114
http://dx.doi.org/10.3389/fcimb.2022.849915
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author Lotoux, Aurélie
Milohanic, Eliane
Bierne, Hélène
author_facet Lotoux, Aurélie
Milohanic, Eliane
Bierne, Hélène
author_sort Lotoux, Aurélie
collection PubMed
description Many bacterial species, including several pathogens, can enter a so-called “viable but non-culturable” (VBNC) state when subjected to stress. Bacteria in the VBNC state are metabolically active but have lost their ability to grow on standard culture media, which compromises their detection by conventional techniques based on bacterial division. Under certain conditions, VBNC bacteria can regain their growth capacity and, for pathogens, their virulence potential, through a process called resuscitation. Here, we review the current state of knowledge of the VBNC state of Listeria monocytogenes (Lm), a Gram-positive pathogenic bacterium responsible for listeriosis, one of the most dangerous foodborne zoonosis. After a brief summary of characteristics of VBNC bacteria, we highlight work on VBNC Lm in the environment and in agricultural and food industry settings, with particular emphasis on the impact of antimicrobial treatments. We subsequently discuss recent data suggesting that Lm can enter the VBNC state in the host, raising the possibility that VBNC forms contribute to the asymptomatic carriage of this pathogen in wildlife, livestock and even humans. We also consider the resuscitation and virulence potential of VBNC Lm and the danger posed by these bacteria to at-risk individuals, particularly pregnant women. Overall, we put forth the hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches in the One-Health continuum, and suggest that screening for healthy carriers, using alternative techniques to culture-based enrichment methods, should better prevent listeriosis risks.
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spelling pubmed-89749162022-04-02 The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum Lotoux, Aurélie Milohanic, Eliane Bierne, Hélène Front Cell Infect Microbiol Cellular and Infection Microbiology Many bacterial species, including several pathogens, can enter a so-called “viable but non-culturable” (VBNC) state when subjected to stress. Bacteria in the VBNC state are metabolically active but have lost their ability to grow on standard culture media, which compromises their detection by conventional techniques based on bacterial division. Under certain conditions, VBNC bacteria can regain their growth capacity and, for pathogens, their virulence potential, through a process called resuscitation. Here, we review the current state of knowledge of the VBNC state of Listeria monocytogenes (Lm), a Gram-positive pathogenic bacterium responsible for listeriosis, one of the most dangerous foodborne zoonosis. After a brief summary of characteristics of VBNC bacteria, we highlight work on VBNC Lm in the environment and in agricultural and food industry settings, with particular emphasis on the impact of antimicrobial treatments. We subsequently discuss recent data suggesting that Lm can enter the VBNC state in the host, raising the possibility that VBNC forms contribute to the asymptomatic carriage of this pathogen in wildlife, livestock and even humans. We also consider the resuscitation and virulence potential of VBNC Lm and the danger posed by these bacteria to at-risk individuals, particularly pregnant women. Overall, we put forth the hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches in the One-Health continuum, and suggest that screening for healthy carriers, using alternative techniques to culture-based enrichment methods, should better prevent listeriosis risks. Frontiers Media S.A. 2022-03-18 /pmc/articles/PMC8974916/ /pubmed/35372114 http://dx.doi.org/10.3389/fcimb.2022.849915 Text en Copyright © 2022 Lotoux, Milohanic and Bierne https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Lotoux, Aurélie
Milohanic, Eliane
Bierne, Hélène
The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum
title The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum
title_full The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum
title_fullStr The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum
title_full_unstemmed The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum
title_short The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum
title_sort viable but non-culturable state of listeria monocytogenes in the one-health continuum
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974916/
https://www.ncbi.nlm.nih.gov/pubmed/35372114
http://dx.doi.org/10.3389/fcimb.2022.849915
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