Cargando…

Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell

Tcf12 has been identified as one of the main helix-loop-helix transcription factors that regulates T cell development from double negative to double positive stage transition. While, the function of Tcf12 in hematopoietic stem cells remains not investigated. In this study, we observed that Tcf12 is...

Descripción completa

Detalles Bibliográficos
Autores principales: Liao, Min, Wang, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974954/
https://www.ncbi.nlm.nih.gov/pubmed/35399207
http://dx.doi.org/10.1097/BS9.0000000000000059
_version_ 1784680308942045184
author Liao, Min
Wang, Jianwei
author_facet Liao, Min
Wang, Jianwei
author_sort Liao, Min
collection PubMed
description Tcf12 has been identified as one of the main helix-loop-helix transcription factors that regulates T cell development from double negative to double positive stage transition. While, the function of Tcf12 in hematopoietic stem cells remains not investigated. In this study, we observed that Tcf12 is expressed in HSCs and targeted deletion of Tcf12 in hematopoietic cells results in increased frequency and absolute number of HSCs, but compromises the reconstitution capacity of HSCs. Further analysis reveals that Tcf12 is dispensable for the self-renewal of HSCs. The declined reconstituted capacity of Tcf12(−/−) HSCs stems from the decrease in the ability to differentiate into lymphoid-primed multipotent progenitors, and furthermore B and T lineages.
format Online
Article
Text
id pubmed-8974954
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-89749542022-04-07 Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell Liao, Min Wang, Jianwei Blood Sci Research Article Tcf12 has been identified as one of the main helix-loop-helix transcription factors that regulates T cell development from double negative to double positive stage transition. While, the function of Tcf12 in hematopoietic stem cells remains not investigated. In this study, we observed that Tcf12 is expressed in HSCs and targeted deletion of Tcf12 in hematopoietic cells results in increased frequency and absolute number of HSCs, but compromises the reconstitution capacity of HSCs. Further analysis reveals that Tcf12 is dispensable for the self-renewal of HSCs. The declined reconstituted capacity of Tcf12(−/−) HSCs stems from the decrease in the ability to differentiate into lymphoid-primed multipotent progenitors, and furthermore B and T lineages. Lippincott Williams & Wilkins 2020-12-01 /pmc/articles/PMC8974954/ /pubmed/35399207 http://dx.doi.org/10.1097/BS9.0000000000000059 Text en Copyright © 2020 The Authors. Published by Wolters Kluwer Health Inc., on behalf of the Chinese Association for Blood Sciences. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Research Article
Liao, Min
Wang, Jianwei
Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
title Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
title_full Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
title_fullStr Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
title_full_unstemmed Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
title_short Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
title_sort tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974954/
https://www.ncbi.nlm.nih.gov/pubmed/35399207
http://dx.doi.org/10.1097/BS9.0000000000000059
work_keys_str_mv AT liaomin tcf12balancesthereconstitutionanddifferentiationcapacityofhematopoieticstemcell
AT wangjianwei tcf12balancesthereconstitutionanddifferentiationcapacityofhematopoieticstemcell