Cargando…

Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum

To understand the behavior and function of bone-marrow mesenchymal cells (BMMCs), we overviewed the morphological presentation of BMMCs in bone-marrow granules (b-BMMCs), isolated BMMCs (i-BMMCs), and BMMCs (c-BMMCs) cultured in H4434 methylcellulose semisolid and MEM media. All samples were derived...

Descripción completa

Detalles Bibliográficos
Autores principales: Ru, Yong-Xin, Dong, Shu-Xu, Xu, Chun-Hui, Zhao, Shi-Xuan, Zhang, Hua-Mei, Liang, Hao-Yue, Fen, Min, Zhang, Feng-Kui, Gao, Ying-Dai, Qi, Shu-Lin, Shang, Hong-Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8975078/
https://www.ncbi.nlm.nih.gov/pubmed/35399204
http://dx.doi.org/10.1097/BS9.0000000000000062
_version_ 1784680327288979456
author Ru, Yong-Xin
Dong, Shu-Xu
Xu, Chun-Hui
Zhao, Shi-Xuan
Zhang, Hua-Mei
Liang, Hao-Yue
Fen, Min
Zhang, Feng-Kui
Gao, Ying-Dai
Qi, Shu-Lin
Shang, Hong-Cai
author_facet Ru, Yong-Xin
Dong, Shu-Xu
Xu, Chun-Hui
Zhao, Shi-Xuan
Zhang, Hua-Mei
Liang, Hao-Yue
Fen, Min
Zhang, Feng-Kui
Gao, Ying-Dai
Qi, Shu-Lin
Shang, Hong-Cai
author_sort Ru, Yong-Xin
collection PubMed
description To understand the behavior and function of bone-marrow mesenchymal cells (BMMCs), we overviewed the morphological presentation of BMMCs in bone-marrow granules (b-BMMCs), isolated BMMCs (i-BMMCs), and BMMCs (c-BMMCs) cultured in H4434 methylcellulose semisolid and MEM media. All samples were derived from bone-marrow aspirates of 30 patients with hematocytopenia. Light microscopy exhibited b-BMMCs and i-BMMCs characterized by abundant cytoplasm and irregular shape in bone-marrow smears, as well as c-BMMCs in culture conditions. Scanning electron microscopy demonstrated cultured c-BMMCs with a sheet-like feature enveloping hematopoietic cells. Transmission electron microscopy revealed b-BMMCs constructing a honeycomb-like structure by thin bifurcate processes among hematopoietic cells. Furthermore, i-BMMCs had bifurcate parapodiums on the surface and prominent rough endoplasmic reticulum (rER) connected with the plasmalemma of the parapodiums. The detailed images suggested that rER may serve as a membrane resource for plasmalemmal expansion in BMMCs in bone marrow.
format Online
Article
Text
id pubmed-8975078
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-89750782022-04-07 Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum Ru, Yong-Xin Dong, Shu-Xu Xu, Chun-Hui Zhao, Shi-Xuan Zhang, Hua-Mei Liang, Hao-Yue Fen, Min Zhang, Feng-Kui Gao, Ying-Dai Qi, Shu-Lin Shang, Hong-Cai Blood Sci Research Article To understand the behavior and function of bone-marrow mesenchymal cells (BMMCs), we overviewed the morphological presentation of BMMCs in bone-marrow granules (b-BMMCs), isolated BMMCs (i-BMMCs), and BMMCs (c-BMMCs) cultured in H4434 methylcellulose semisolid and MEM media. All samples were derived from bone-marrow aspirates of 30 patients with hematocytopenia. Light microscopy exhibited b-BMMCs and i-BMMCs characterized by abundant cytoplasm and irregular shape in bone-marrow smears, as well as c-BMMCs in culture conditions. Scanning electron microscopy demonstrated cultured c-BMMCs with a sheet-like feature enveloping hematopoietic cells. Transmission electron microscopy revealed b-BMMCs constructing a honeycomb-like structure by thin bifurcate processes among hematopoietic cells. Furthermore, i-BMMCs had bifurcate parapodiums on the surface and prominent rough endoplasmic reticulum (rER) connected with the plasmalemma of the parapodiums. The detailed images suggested that rER may serve as a membrane resource for plasmalemmal expansion in BMMCs in bone marrow. Lippincott Williams & Wilkins 2020-11-17 /pmc/articles/PMC8975078/ /pubmed/35399204 http://dx.doi.org/10.1097/BS9.0000000000000062 Text en Copyright © 2020 The Authors. Published by Wolters Kluwer Health Inc., on behalf of the Chinese Association for Blood Sciences. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Research Article
Ru, Yong-Xin
Dong, Shu-Xu
Xu, Chun-Hui
Zhao, Shi-Xuan
Zhang, Hua-Mei
Liang, Hao-Yue
Fen, Min
Zhang, Feng-Kui
Gao, Ying-Dai
Qi, Shu-Lin
Shang, Hong-Cai
Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
title Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
title_full Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
title_fullStr Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
title_full_unstemmed Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
title_short Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
title_sort bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8975078/
https://www.ncbi.nlm.nih.gov/pubmed/35399204
http://dx.doi.org/10.1097/BS9.0000000000000062
work_keys_str_mv AT ruyongxin bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT dongshuxu bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT xuchunhui bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT zhaoshixuan bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT zhanghuamei bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT lianghaoyue bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT fenmin bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT zhangfengkui bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT gaoyingdai bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT qishulin bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum
AT shanghongcai bonemarrowmesenchymalcellspolymorphismassociatedwithtransformationofroughendoplasmicreticulum