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ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death
Sexual plant reproduction depends on the attraction of sperm-cell delivering pollen tubes (PT) by two synergids, followed by their programmed cell death (PCD) in Arabidopsis. Disruption of the mitogen-activated protein kinase 4 (MPK4) by pathogenic effectors activates the resistance protein (R) SUMM...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976062/ https://www.ncbi.nlm.nih.gov/pubmed/35365652 http://dx.doi.org/10.1038/s41467-022-29373-7 |
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author | Völz, Ronny Harris, William Hirt, Heribert Lee, Yong-Hwan |
author_facet | Völz, Ronny Harris, William Hirt, Heribert Lee, Yong-Hwan |
author_sort | Völz, Ronny |
collection | PubMed |
description | Sexual plant reproduction depends on the attraction of sperm-cell delivering pollen tubes (PT) by two synergids, followed by their programmed cell death (PCD) in Arabidopsis. Disruption of the mitogen-activated protein kinase 4 (MPK4) by pathogenic effectors activates the resistance protein (R) SUMM2-mediated immunity and cell death. Here we show that synergid preservation and reactive oxygen species (ROS) homeostasis are intimately linked and maintained by MPK4. In mpk4, ROS levels are increased and synergids prematurely undergo PCD before PT-reception. However, ROS scavengers and the disruption of SUMM2, in mpk4, restore ROS homeostasis, synergid maintenance and PT perception, demonstrating that the guardian of MPK4, SUMM2, triggers synergid-PCD. In mpk4/summ2, PTs show a feronia-like overgrowth phenotype. Our results show that immunity-associated PCD and synergid cell death during plant reproduction are regulated by MPK4 underscoring an underlying molecular mechanism for the suppression of plant reproduction during systemic R-mediated immunity. |
format | Online Article Text |
id | pubmed-8976062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89760622022-04-20 ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death Völz, Ronny Harris, William Hirt, Heribert Lee, Yong-Hwan Nat Commun Article Sexual plant reproduction depends on the attraction of sperm-cell delivering pollen tubes (PT) by two synergids, followed by their programmed cell death (PCD) in Arabidopsis. Disruption of the mitogen-activated protein kinase 4 (MPK4) by pathogenic effectors activates the resistance protein (R) SUMM2-mediated immunity and cell death. Here we show that synergid preservation and reactive oxygen species (ROS) homeostasis are intimately linked and maintained by MPK4. In mpk4, ROS levels are increased and synergids prematurely undergo PCD before PT-reception. However, ROS scavengers and the disruption of SUMM2, in mpk4, restore ROS homeostasis, synergid maintenance and PT perception, demonstrating that the guardian of MPK4, SUMM2, triggers synergid-PCD. In mpk4/summ2, PTs show a feronia-like overgrowth phenotype. Our results show that immunity-associated PCD and synergid cell death during plant reproduction are regulated by MPK4 underscoring an underlying molecular mechanism for the suppression of plant reproduction during systemic R-mediated immunity. Nature Publishing Group UK 2022-04-01 /pmc/articles/PMC8976062/ /pubmed/35365652 http://dx.doi.org/10.1038/s41467-022-29373-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Völz, Ronny Harris, William Hirt, Heribert Lee, Yong-Hwan ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death |
title | ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death |
title_full | ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death |
title_fullStr | ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death |
title_full_unstemmed | ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death |
title_short | ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death |
title_sort | ros homeostasis mediated by mpk4 and summ2 determines synergid cell death |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976062/ https://www.ncbi.nlm.nih.gov/pubmed/35365652 http://dx.doi.org/10.1038/s41467-022-29373-7 |
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