Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

BACKGROUND: The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure for the simultaneous detection of DNA and RNA p...

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Detalles Bibliográficos
Autores principales: Jiang, Hanfang, Xing, Zhihao, Liu, Xiaorong, Chai, Qiang, Xin, Zefeng, Zhu, Chunqing, Lin, Ruihong, Deng, Xuwen, Cui, Dong, Gao, HongDan, Ma, Dongli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976360/
https://www.ncbi.nlm.nih.gov/pubmed/35365081
http://dx.doi.org/10.1186/s12879-022-07272-y

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