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Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells

OBJECTIVE(S): Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we i...

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Autores principales: Kazemi, Mohaddeseh, Kouhpeikar, Hamideh, Delbari, Zahra, Khodadadi, Faeze, Gerayli, Sina, Iranshahi, Mehrdad, Mosavat, Arman, Behnam Rassouli, Fatemeh, Rafatpanah, Houshang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976908/
https://www.ncbi.nlm.nih.gov/pubmed/35432798
http://dx.doi.org/10.22038/IJBMS.2021.58633.13025
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author Kazemi, Mohaddeseh
Kouhpeikar, Hamideh
Delbari, Zahra
Khodadadi, Faeze
Gerayli, Sina
Iranshahi, Mehrdad
Mosavat, Arman
Behnam Rassouli, Fatemeh
Rafatpanah, Houshang
author_facet Kazemi, Mohaddeseh
Kouhpeikar, Hamideh
Delbari, Zahra
Khodadadi, Faeze
Gerayli, Sina
Iranshahi, Mehrdad
Mosavat, Arman
Behnam Rassouli, Fatemeh
Rafatpanah, Houshang
author_sort Kazemi, Mohaddeseh
collection PubMed
description OBJECTIVE(S): Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we investigated the combinatorial effects of AUR and arsenic trioxide (ATO) on MT-2 cells. MATERIALS AND METHODS: The cells were treated with different concentrations of AUR for 24, 48, and 72 hr, and viability was measured by alamarBlue assay. Then, the combination of AUR (20 μg/ml) and ATO (3 μg/ml) was administrated and the cell cycle was analyzed by PI staining followed by flow cytometry analysis. In addition, the expression of NF-κB (REL-A), CD44, c-MYC, and BMI-1 was evaluated via qPCR. RESULTS: Assessment of cell viability revealed increased toxicity of AUR and ATO when used in combination. Our findings were confirmed by accumulation of cells in the sub G1 phase of the cell cycle and significant down-regulation of NF-κB (REL-A), CD44, c-MYC, and BMI-1. CONCLUSION: Obtained findings suggest that combinatorial use of AUR and ATO could be considered for designing novel chemotherapy regimens for ATLL.
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spelling pubmed-89769082022-04-14 Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells Kazemi, Mohaddeseh Kouhpeikar, Hamideh Delbari, Zahra Khodadadi, Faeze Gerayli, Sina Iranshahi, Mehrdad Mosavat, Arman Behnam Rassouli, Fatemeh Rafatpanah, Houshang Iran J Basic Med Sci Original Article OBJECTIVE(S): Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we investigated the combinatorial effects of AUR and arsenic trioxide (ATO) on MT-2 cells. MATERIALS AND METHODS: The cells were treated with different concentrations of AUR for 24, 48, and 72 hr, and viability was measured by alamarBlue assay. Then, the combination of AUR (20 μg/ml) and ATO (3 μg/ml) was administrated and the cell cycle was analyzed by PI staining followed by flow cytometry analysis. In addition, the expression of NF-κB (REL-A), CD44, c-MYC, and BMI-1 was evaluated via qPCR. RESULTS: Assessment of cell viability revealed increased toxicity of AUR and ATO when used in combination. Our findings were confirmed by accumulation of cells in the sub G1 phase of the cell cycle and significant down-regulation of NF-κB (REL-A), CD44, c-MYC, and BMI-1. CONCLUSION: Obtained findings suggest that combinatorial use of AUR and ATO could be considered for designing novel chemotherapy regimens for ATLL. Mashhad University of Medical Sciences 2021-12 /pmc/articles/PMC8976908/ /pubmed/35432798 http://dx.doi.org/10.22038/IJBMS.2021.58633.13025 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kazemi, Mohaddeseh
Kouhpeikar, Hamideh
Delbari, Zahra
Khodadadi, Faeze
Gerayli, Sina
Iranshahi, Mehrdad
Mosavat, Arman
Behnam Rassouli, Fatemeh
Rafatpanah, Houshang
Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
title Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
title_full Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
title_fullStr Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
title_full_unstemmed Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
title_short Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
title_sort combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subg1 phase in adult t-cell leukemia cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976908/
https://www.ncbi.nlm.nih.gov/pubmed/35432798
http://dx.doi.org/10.22038/IJBMS.2021.58633.13025
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