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Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila

BACKGROUND: Ophiorrhiza pumila (Rubiaceae) is capable of producing camptothecin (CPT), one monoterpene indole alkaloid extensively employed in the treatment of multiple cancers. Transcription factors (TFs) GATA are a group of transcription regulators involved in plant development and metabolism, and...

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Autores principales: Shi, Min, Huang, Qikai, Wang, Yao, Wang, Can, Zhu, Ruiyan, Zhang, Siwei, Kai, Guoyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977026/
https://www.ncbi.nlm.nih.gov/pubmed/35366818
http://dx.doi.org/10.1186/s12864-022-08484-x
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author Shi, Min
Huang, Qikai
Wang, Yao
Wang, Can
Zhu, Ruiyan
Zhang, Siwei
Kai, Guoyin
author_facet Shi, Min
Huang, Qikai
Wang, Yao
Wang, Can
Zhu, Ruiyan
Zhang, Siwei
Kai, Guoyin
author_sort Shi, Min
collection PubMed
description BACKGROUND: Ophiorrhiza pumila (Rubiaceae) is capable of producing camptothecin (CPT), one monoterpene indole alkaloid extensively employed in the treatment of multiple cancers. Transcription factors (TFs) GATA are a group of transcription regulators involved in plant development and metabolism, and show the feature of binding to the GATA motif within the promoters of target genes. However, GATA TFs have not been characterized in O. pumila. RESULT: In this study, a total of 18 GATA genes classified into four subfamilies were identified, which randomly distributed on 11 chromosomes of O. pumila. Synteny analysis of GATA genes between O. pumila and other plant species such as Arabidopsis thaliana, Oryza sativa, Glycine max, Solanum lycopersicum, Vitis vinifera, and Catharanthus roseus genomes were analyzed. Tissue expression pattern revealed that OpGATA1 and OpGATA18 were found to be correlated with ASA, MK, CPR and GPPS, which were highly expressed in leaves. OpGATA7, showed high expression in roots as most of the CPT biosynthetic pathway genes did, suggesting that these OpGATAs may be potential candidates regulating CPT biosynthesis in O. pumila. CONCLUSIONS: In this study, we systematically analyzed the OpGATA TFs, and provided insights into the involvement of OpGATA TFs from O. pumila in CPT biosynthesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08484-x.
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spelling pubmed-89770262022-04-04 Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila Shi, Min Huang, Qikai Wang, Yao Wang, Can Zhu, Ruiyan Zhang, Siwei Kai, Guoyin BMC Genomics Research BACKGROUND: Ophiorrhiza pumila (Rubiaceae) is capable of producing camptothecin (CPT), one monoterpene indole alkaloid extensively employed in the treatment of multiple cancers. Transcription factors (TFs) GATA are a group of transcription regulators involved in plant development and metabolism, and show the feature of binding to the GATA motif within the promoters of target genes. However, GATA TFs have not been characterized in O. pumila. RESULT: In this study, a total of 18 GATA genes classified into four subfamilies were identified, which randomly distributed on 11 chromosomes of O. pumila. Synteny analysis of GATA genes between O. pumila and other plant species such as Arabidopsis thaliana, Oryza sativa, Glycine max, Solanum lycopersicum, Vitis vinifera, and Catharanthus roseus genomes were analyzed. Tissue expression pattern revealed that OpGATA1 and OpGATA18 were found to be correlated with ASA, MK, CPR and GPPS, which were highly expressed in leaves. OpGATA7, showed high expression in roots as most of the CPT biosynthetic pathway genes did, suggesting that these OpGATAs may be potential candidates regulating CPT biosynthesis in O. pumila. CONCLUSIONS: In this study, we systematically analyzed the OpGATA TFs, and provided insights into the involvement of OpGATA TFs from O. pumila in CPT biosynthesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08484-x. BioMed Central 2022-04-03 /pmc/articles/PMC8977026/ /pubmed/35366818 http://dx.doi.org/10.1186/s12864-022-08484-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Shi, Min
Huang, Qikai
Wang, Yao
Wang, Can
Zhu, Ruiyan
Zhang, Siwei
Kai, Guoyin
Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila
title Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila
title_full Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila
title_fullStr Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila
title_full_unstemmed Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila
title_short Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila
title_sort genome-wide survey of the gata gene family in camptothecin-producing plant ophiorrhiza pumila
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977026/
https://www.ncbi.nlm.nih.gov/pubmed/35366818
http://dx.doi.org/10.1186/s12864-022-08484-x
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