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Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling
BACKGROUND: Pathological retinal angiogenesis resulting from a variety of ocular diseases including oxygen induced retinopathy, diabetic retinopathy and ocular vein occlusion, is one of the major reasons for vision loss, yet the therapeutic option is limited. Multiple nanoparticles have been reporte...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977040/ https://www.ncbi.nlm.nih.gov/pubmed/35366885 http://dx.doi.org/10.1186/s12951-022-01362-4 |
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author | Zhao, Na Gui, Xiao Fang, Qian Zhang, Rui Zhu, Weiye Zhang, Haorui Li, Qing Zhou, Yukun Zhao, Jiawei Cui, Xiao Gao, Guangping Tang, Huipeng Shen, Ni Chen, Taoyong Song, Hongyuan Shen, Wei |
author_facet | Zhao, Na Gui, Xiao Fang, Qian Zhang, Rui Zhu, Weiye Zhang, Haorui Li, Qing Zhou, Yukun Zhao, Jiawei Cui, Xiao Gao, Guangping Tang, Huipeng Shen, Ni Chen, Taoyong Song, Hongyuan Shen, Wei |
author_sort | Zhao, Na |
collection | PubMed |
description | BACKGROUND: Pathological retinal angiogenesis resulting from a variety of ocular diseases including oxygen induced retinopathy, diabetic retinopathy and ocular vein occlusion, is one of the major reasons for vision loss, yet the therapeutic option is limited. Multiple nanoparticles have been reported to alleviate angiogenic retinopathy. However, the adverse effect cannot be ignored due to the relatively large scale. Graphene quantum dots (GQDs) have shown potential in drug delivery and have been proved biocompatible. In this study, Graphene quantum dots are extensively investigated for their application in angiogenic retinopathy therapy. RESULTS: We showed that GQDs were biocompatible nanomaterials in vitro and in vivo. The nanoparticles have a dose-dependent inhibitory effect on proliferation, migration, tube formation and sprouting of human umbilical vein endothelial cells (HUVECs). Further data show that GQDs could inhibit pathological retinal neovascularization in an oxygen-induced retinopathy (OIR) model. The data of RNA sequencing suggested that periostin is involved in this process. GQDs inhibit the expression of periostin via STAT3, and further regulated cell cycle-related protein levels through ERK pathway. The signaling pathway was conformed in vivo using OIR mouse model. CONCLUSIONS: The present study indicated that GQDs could be a biocompatible anti-angiogenic nanomedicine in the treatment of pathological retinal neovascularization via disrupting periostin/ERK pathway and subsequent cell cycle. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01362-4. |
format | Online Article Text |
id | pubmed-8977040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-89770402022-04-04 Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling Zhao, Na Gui, Xiao Fang, Qian Zhang, Rui Zhu, Weiye Zhang, Haorui Li, Qing Zhou, Yukun Zhao, Jiawei Cui, Xiao Gao, Guangping Tang, Huipeng Shen, Ni Chen, Taoyong Song, Hongyuan Shen, Wei J Nanobiotechnology Research BACKGROUND: Pathological retinal angiogenesis resulting from a variety of ocular diseases including oxygen induced retinopathy, diabetic retinopathy and ocular vein occlusion, is one of the major reasons for vision loss, yet the therapeutic option is limited. Multiple nanoparticles have been reported to alleviate angiogenic retinopathy. However, the adverse effect cannot be ignored due to the relatively large scale. Graphene quantum dots (GQDs) have shown potential in drug delivery and have been proved biocompatible. In this study, Graphene quantum dots are extensively investigated for their application in angiogenic retinopathy therapy. RESULTS: We showed that GQDs were biocompatible nanomaterials in vitro and in vivo. The nanoparticles have a dose-dependent inhibitory effect on proliferation, migration, tube formation and sprouting of human umbilical vein endothelial cells (HUVECs). Further data show that GQDs could inhibit pathological retinal neovascularization in an oxygen-induced retinopathy (OIR) model. The data of RNA sequencing suggested that periostin is involved in this process. GQDs inhibit the expression of periostin via STAT3, and further regulated cell cycle-related protein levels through ERK pathway. The signaling pathway was conformed in vivo using OIR mouse model. CONCLUSIONS: The present study indicated that GQDs could be a biocompatible anti-angiogenic nanomedicine in the treatment of pathological retinal neovascularization via disrupting periostin/ERK pathway and subsequent cell cycle. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01362-4. BioMed Central 2022-04-02 /pmc/articles/PMC8977040/ /pubmed/35366885 http://dx.doi.org/10.1186/s12951-022-01362-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhao, Na Gui, Xiao Fang, Qian Zhang, Rui Zhu, Weiye Zhang, Haorui Li, Qing Zhou, Yukun Zhao, Jiawei Cui, Xiao Gao, Guangping Tang, Huipeng Shen, Ni Chen, Taoyong Song, Hongyuan Shen, Wei Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling |
title | Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling |
title_full | Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling |
title_fullStr | Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling |
title_full_unstemmed | Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling |
title_short | Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling |
title_sort | graphene quantum dots rescue angiogenic retinopathy via blocking stat3/periostin/erk signaling |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977040/ https://www.ncbi.nlm.nih.gov/pubmed/35366885 http://dx.doi.org/10.1186/s12951-022-01362-4 |
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