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Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension

Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes (DM2). Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, n...

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Autores principales: Milutinović, Aleksandra, Zorc-Pleskovič, Ruda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977081/
https://www.ncbi.nlm.nih.gov/pubmed/34656077
http://dx.doi.org/10.17305/bjbms.2021.6488
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author Milutinović, Aleksandra
Zorc-Pleskovič, Ruda
author_facet Milutinović, Aleksandra
Zorc-Pleskovič, Ruda
author_sort Milutinović, Aleksandra
collection PubMed
description Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes (DM2). Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, n = 6) versus hypertensive non-diabetic patients (AH group, n = 34). For histologic analysis, the sections were stained with hematoxylin-eosin and Movat pentachrome. The immunohistochemical staining was used to analyze the infiltration of pro-inflammatory (CD68) and anti-inflammatory macrophages (CD163), T helper (CD4) and T killer cells (CD8), and B (CD79a) and plasma cells (CD138) in all three layers of aneurysms of both groups. The statistical significance of the differences between groups was evaluated by ANOVA and the Welch test. In comparison to the AH group, the DM2 group developed less severe infiltration of pro-inflammatory macrophages (p = 0.004) and B cells (p = 0.025) in the tunica intima and tunica media (p = 0.049 and p = 0.007, respectively), and fewer plasma cells in the tunica media (p = 0.024) and tunica adventitia (p = 0.017). We found no significant differences in the number of T helper, T killer cells, and anti-inflammatory macrophages and in the amount of collagen and elastic fibers, ground substance, and smooth muscle cells in all three layers of the vessel wall. Except in tunica adventitia of DM2 group, there were more collagen fibers overall (p = 0.025). Thus, we conclude that the histological structure of the aneurysm in diabetics without hypertension is almost the same as in hypertensive patients without diabetes. Diabetics had significantly less inflammatory infiltration in all three layers of the vessel wall and more collagen fibers in tunica adventitia.
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spelling pubmed-89770812022-04-14 Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension Milutinović, Aleksandra Zorc-Pleskovič, Ruda Bosn J Basic Med Sci Research Article Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes (DM2). Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, n = 6) versus hypertensive non-diabetic patients (AH group, n = 34). For histologic analysis, the sections were stained with hematoxylin-eosin and Movat pentachrome. The immunohistochemical staining was used to analyze the infiltration of pro-inflammatory (CD68) and anti-inflammatory macrophages (CD163), T helper (CD4) and T killer cells (CD8), and B (CD79a) and plasma cells (CD138) in all three layers of aneurysms of both groups. The statistical significance of the differences between groups was evaluated by ANOVA and the Welch test. In comparison to the AH group, the DM2 group developed less severe infiltration of pro-inflammatory macrophages (p = 0.004) and B cells (p = 0.025) in the tunica intima and tunica media (p = 0.049 and p = 0.007, respectively), and fewer plasma cells in the tunica media (p = 0.024) and tunica adventitia (p = 0.017). We found no significant differences in the number of T helper, T killer cells, and anti-inflammatory macrophages and in the amount of collagen and elastic fibers, ground substance, and smooth muscle cells in all three layers of the vessel wall. Except in tunica adventitia of DM2 group, there were more collagen fibers overall (p = 0.025). Thus, we conclude that the histological structure of the aneurysm in diabetics without hypertension is almost the same as in hypertensive patients without diabetes. Diabetics had significantly less inflammatory infiltration in all three layers of the vessel wall and more collagen fibers in tunica adventitia. Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2022-04 2021-10-12 /pmc/articles/PMC8977081/ /pubmed/34656077 http://dx.doi.org/10.17305/bjbms.2021.6488 Text en Copyright: © The Author(s) (2022) https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License
spellingShingle Research Article
Milutinović, Aleksandra
Zorc-Pleskovič, Ruda
Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
title Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
title_full Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
title_fullStr Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
title_full_unstemmed Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
title_short Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
title_sort inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977081/
https://www.ncbi.nlm.nih.gov/pubmed/34656077
http://dx.doi.org/10.17305/bjbms.2021.6488
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