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Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide
BACKGROUND: Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflamm...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Pain Society
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977206/ https://www.ncbi.nlm.nih.gov/pubmed/35354677 http://dx.doi.org/10.3344/kjp.2022.35.2.140 |
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author | Moghrovyan, Armenuhi Parseghyan, Lilya Sevoyan, Gohar Darbinyan, Anna Sahakyan, Naira Gaboyan, Monica Karabekian, Zaruhi Voskanyan, Armen |
author_facet | Moghrovyan, Armenuhi Parseghyan, Lilya Sevoyan, Gohar Darbinyan, Anna Sahakyan, Naira Gaboyan, Monica Karabekian, Zaruhi Voskanyan, Armen |
author_sort | Moghrovyan, Armenuhi |
collection | PubMed |
description | BACKGROUND: Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation. METHODS: For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used. RESULTS: The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media. CONCLUSIONS: The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations. |
format | Online Article Text |
id | pubmed-8977206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Pain Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-89772062022-04-07 Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide Moghrovyan, Armenuhi Parseghyan, Lilya Sevoyan, Gohar Darbinyan, Anna Sahakyan, Naira Gaboyan, Monica Karabekian, Zaruhi Voskanyan, Armen Korean J Pain Experimental Research Articles BACKGROUND: Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation. METHODS: For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used. RESULTS: The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media. CONCLUSIONS: The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations. The Korean Pain Society 2022-04-01 2022-04-01 /pmc/articles/PMC8977206/ /pubmed/35354677 http://dx.doi.org/10.3344/kjp.2022.35.2.140 Text en © The Korean Pain Society, 2022 https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Experimental Research Articles Moghrovyan, Armenuhi Parseghyan, Lilya Sevoyan, Gohar Darbinyan, Anna Sahakyan, Naira Gaboyan, Monica Karabekian, Zaruhi Voskanyan, Armen Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
title | Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
title_full | Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
title_fullStr | Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
title_full_unstemmed | Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
title_short | Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
title_sort | antinociceptive, anti-inflammatory, and cytotoxic properties of origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide |
topic | Experimental Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977206/ https://www.ncbi.nlm.nih.gov/pubmed/35354677 http://dx.doi.org/10.3344/kjp.2022.35.2.140 |
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