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Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion

Since April 2021, the plasma aldosterone concentration has been measured by chemiluminescent enzyme immunoassay (CLEIA) in Japan. In the present study, we developed a new CLEIA using a two-step sandwich method to measure the 24-hour urine aldosterone level. We collected 115 urine samples and measure...

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Autores principales: Ozeki, Yoshinori, Kinoshita, Mizuki, Miyamoto, Shotaro, Yoshida, Yuichi, Okamoto, Mitsuhiro, Gotoh, Koro, Masaki, Takayuki, Kambara, Kengo, Shibata, Hirotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977523/
https://www.ncbi.nlm.nih.gov/pubmed/35388294
http://dx.doi.org/10.3389/fendo.2022.859347
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author Ozeki, Yoshinori
Kinoshita, Mizuki
Miyamoto, Shotaro
Yoshida, Yuichi
Okamoto, Mitsuhiro
Gotoh, Koro
Masaki, Takayuki
Kambara, Kengo
Shibata, Hirotaka
author_facet Ozeki, Yoshinori
Kinoshita, Mizuki
Miyamoto, Shotaro
Yoshida, Yuichi
Okamoto, Mitsuhiro
Gotoh, Koro
Masaki, Takayuki
Kambara, Kengo
Shibata, Hirotaka
author_sort Ozeki, Yoshinori
collection PubMed
description Since April 2021, the plasma aldosterone concentration has been measured by chemiluminescent enzyme immunoassay (CLEIA) in Japan. In the present study, we developed a new CLEIA using a two-step sandwich method to measure the 24-hour urine aldosterone level. We collected 115 urine samples and measured 24-hour urine aldosterone levels employing radioimmunoassay (RIA), CLEIA, and liquid chromatography–tandem mass spectrometry (LC-MS/MS). The results showed that the 24-hour urine aldosterone levels measured using CLEIA and LC-MS/MS were significantly correlated (ρ = 0.992, P < 0.0001). Based on the results of Passing–Bablok regression analysis, the slope was 0.992 and the intercept –19.3. The 24-hour urine aldosterone levels measured using CLEIA and RIA were also significantly correlated (ρ = 0.905, P < 0.0001). However, the aldosterone level measured by CLEIA was lower than that measured by RIA (slope, 0.729; intercept, 120.9). In Japan, a new guideline for primary aldosteronism has been announced, with changes in the aldosterone measurement method. The cutoff values for oral sodium loading test (OSLT) were changed, but clinical verification using real-world urine samples has not been performed. Therefore, we examined the cut-off value of the 24-hour urine aldosterone level after the OSLT. Receiver operating characteristic analysis revealed a cut-off value for primary aldosteronism of 3 μg/day.
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spelling pubmed-89775232022-04-05 Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion Ozeki, Yoshinori Kinoshita, Mizuki Miyamoto, Shotaro Yoshida, Yuichi Okamoto, Mitsuhiro Gotoh, Koro Masaki, Takayuki Kambara, Kengo Shibata, Hirotaka Front Endocrinol (Lausanne) Endocrinology Since April 2021, the plasma aldosterone concentration has been measured by chemiluminescent enzyme immunoassay (CLEIA) in Japan. In the present study, we developed a new CLEIA using a two-step sandwich method to measure the 24-hour urine aldosterone level. We collected 115 urine samples and measured 24-hour urine aldosterone levels employing radioimmunoassay (RIA), CLEIA, and liquid chromatography–tandem mass spectrometry (LC-MS/MS). The results showed that the 24-hour urine aldosterone levels measured using CLEIA and LC-MS/MS were significantly correlated (ρ = 0.992, P < 0.0001). Based on the results of Passing–Bablok regression analysis, the slope was 0.992 and the intercept –19.3. The 24-hour urine aldosterone levels measured using CLEIA and RIA were also significantly correlated (ρ = 0.905, P < 0.0001). However, the aldosterone level measured by CLEIA was lower than that measured by RIA (slope, 0.729; intercept, 120.9). In Japan, a new guideline for primary aldosteronism has been announced, with changes in the aldosterone measurement method. The cutoff values for oral sodium loading test (OSLT) were changed, but clinical verification using real-world urine samples has not been performed. Therefore, we examined the cut-off value of the 24-hour urine aldosterone level after the OSLT. Receiver operating characteristic analysis revealed a cut-off value for primary aldosteronism of 3 μg/day. Frontiers Media S.A. 2022-03-21 /pmc/articles/PMC8977523/ /pubmed/35388294 http://dx.doi.org/10.3389/fendo.2022.859347 Text en Copyright © 2022 Ozeki, Kinoshita, Miyamoto, Yoshida, Okamoto, Gotoh, Masaki, Kambara and Shibata https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Ozeki, Yoshinori
Kinoshita, Mizuki
Miyamoto, Shotaro
Yoshida, Yuichi
Okamoto, Mitsuhiro
Gotoh, Koro
Masaki, Takayuki
Kambara, Kengo
Shibata, Hirotaka
Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion
title Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion
title_full Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion
title_fullStr Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion
title_full_unstemmed Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion
title_short Re-Assessment of the Oral Salt Loading Test Using a New Chemiluminescent Enzyme Immunoassay Based on a Two-Step Sandwich Method to Measure 24-Hour Urine Aldosterone Excretion
title_sort re-assessment of the oral salt loading test using a new chemiluminescent enzyme immunoassay based on a two-step sandwich method to measure 24-hour urine aldosterone excretion
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977523/
https://www.ncbi.nlm.nih.gov/pubmed/35388294
http://dx.doi.org/10.3389/fendo.2022.859347
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