Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry

Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under sy...

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Autores principales: Mo, Jongseo, Angelichio, Michael, Gow, Lisa, Leathers, Valerie, Jackwood, Mark W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977536/
https://www.ncbi.nlm.nih.gov/pubmed/35187879
http://dx.doi.org/10.4142/jvs.21153
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author Mo, Jongseo
Angelichio, Michael
Gow, Lisa
Leathers, Valerie
Jackwood, Mark W.
author_facet Mo, Jongseo
Angelichio, Michael
Gow, Lisa
Leathers, Valerie
Jackwood, Mark W.
author_sort Mo, Jongseo
collection PubMed
description Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 log(10) dynamic range, a reproducible (R(2) > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%–98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.
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spelling pubmed-89775362022-04-11 Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry Mo, Jongseo Angelichio, Michael Gow, Lisa Leathers, Valerie Jackwood, Mark W. J Vet Sci Rapid Communication Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 log(10) dynamic range, a reproducible (R(2) > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%–98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories. The Korean Society of Veterinary Science 2022-01-10 /pmc/articles/PMC8977536/ /pubmed/35187879 http://dx.doi.org/10.4142/jvs.21153 Text en © 2022 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Rapid Communication
Mo, Jongseo
Angelichio, Michael
Gow, Lisa
Leathers, Valerie
Jackwood, Mark W.
Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
title Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
title_full Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
title_fullStr Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
title_full_unstemmed Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
title_short Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
title_sort quantitative real-time pcr assays for the concurrent diagnosis of infectious laryngotracheitis virus, newcastle disease virus and avian metapneumovirus in poultry
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977536/
https://www.ncbi.nlm.nih.gov/pubmed/35187879
http://dx.doi.org/10.4142/jvs.21153
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