Cargando…
Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs
BACKGROUND: The testis has been reported to be a naturally O(2)-deprived organ, dimethyloxaloylglycine (DMOG) can inhibit hypoxia inducible factor-1alpha (HIF-1α) subject to degradation under normal oxygen condition in cells. OBJECTIVES: The objective of this study is to detect the effects of DMOG o...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977544/ https://www.ncbi.nlm.nih.gov/pubmed/35363442 http://dx.doi.org/10.4142/jvs.21308 |
_version_ | 1784680788419149824 |
---|---|
author | Cao, Yaqi Dai, ZiFu Lao, Huizhen Zhao, Huimin |
author_facet | Cao, Yaqi Dai, ZiFu Lao, Huizhen Zhao, Huimin |
author_sort | Cao, Yaqi |
collection | PubMed |
description | BACKGROUND: The testis has been reported to be a naturally O(2)-deprived organ, dimethyloxaloylglycine (DMOG) can inhibit hypoxia inducible factor-1alpha (HIF-1α) subject to degradation under normal oxygen condition in cells. OBJECTIVES: The objective of this study is to detect the effects of DMOG on the proliferation and differentiation of spermatogonial stem cells (SSCs) in Bama minipigs. METHODS: Gradient concentrations of DMOG were added into the culture medium, HIF-1α protein in SSCs was detected by western blot analysis, the relative transcription levels of the SSC-specific genes were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Six days post-induction, the genes related to spermatogenesis were detected by qRT-PCR, and the DNA content was determined by flow cytometry. RESULTS: Results revealed that the levels of HIF-1α protein increased in SSCs with the DMOG treatment in a dose-dependent manner. The relative transcription levels of SSC-specific genes were significantly upregulated (p < 0.05) by activating HIF-1α expression. The induction results showed that DMOG significantly increased (p < 0.05) the spermatogenesis capability of SSCs, and the populations of haploid cells significantly increased (p < 0.05) in DMOG-treated SSCs when compared to those in DMOG-untreated SSCs. CONCLUSION: We demonstrate that DMOG can promote the spermatogenesis activity of SSCs. |
format | Online Article Text |
id | pubmed-8977544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89775442022-04-11 Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs Cao, Yaqi Dai, ZiFu Lao, Huizhen Zhao, Huimin J Vet Sci Original Article BACKGROUND: The testis has been reported to be a naturally O(2)-deprived organ, dimethyloxaloylglycine (DMOG) can inhibit hypoxia inducible factor-1alpha (HIF-1α) subject to degradation under normal oxygen condition in cells. OBJECTIVES: The objective of this study is to detect the effects of DMOG on the proliferation and differentiation of spermatogonial stem cells (SSCs) in Bama minipigs. METHODS: Gradient concentrations of DMOG were added into the culture medium, HIF-1α protein in SSCs was detected by western blot analysis, the relative transcription levels of the SSC-specific genes were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Six days post-induction, the genes related to spermatogenesis were detected by qRT-PCR, and the DNA content was determined by flow cytometry. RESULTS: Results revealed that the levels of HIF-1α protein increased in SSCs with the DMOG treatment in a dose-dependent manner. The relative transcription levels of SSC-specific genes were significantly upregulated (p < 0.05) by activating HIF-1α expression. The induction results showed that DMOG significantly increased (p < 0.05) the spermatogenesis capability of SSCs, and the populations of haploid cells significantly increased (p < 0.05) in DMOG-treated SSCs when compared to those in DMOG-untreated SSCs. CONCLUSION: We demonstrate that DMOG can promote the spermatogenesis activity of SSCs. The Korean Society of Veterinary Science 2022-02-14 /pmc/articles/PMC8977544/ /pubmed/35363442 http://dx.doi.org/10.4142/jvs.21308 Text en © 2022 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cao, Yaqi Dai, ZiFu Lao, Huizhen Zhao, Huimin Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs |
title | Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs |
title_full | Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs |
title_fullStr | Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs |
title_full_unstemmed | Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs |
title_short | Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs |
title_sort | dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in bama minipigs |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977544/ https://www.ncbi.nlm.nih.gov/pubmed/35363442 http://dx.doi.org/10.4142/jvs.21308 |
work_keys_str_mv | AT caoyaqi dimethyloxaloylglycinepromotesspermatogenesisactivityofspermatogonialstemcellsinbamaminipigs AT daizifu dimethyloxaloylglycinepromotesspermatogenesisactivityofspermatogonialstemcellsinbamaminipigs AT laohuizhen dimethyloxaloylglycinepromotesspermatogenesisactivityofspermatogonialstemcellsinbamaminipigs AT zhaohuimin dimethyloxaloylglycinepromotesspermatogenesisactivityofspermatogonialstemcellsinbamaminipigs |