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Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate, and oval. Such pleomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to t...

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Autores principales: Chuberre, Coralie, Chan, Philippe, Walet-Balieu, Marie-Laure, Thiébert, François, Burel, Carole, Hardouin, Julie, Gügi, Bruno, Bardor, Muriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977783/
https://www.ncbi.nlm.nih.gov/pubmed/35386671
http://dx.doi.org/10.3389/fpls.2022.673113
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author Chuberre, Coralie
Chan, Philippe
Walet-Balieu, Marie-Laure
Thiébert, François
Burel, Carole
Hardouin, Julie
Gügi, Bruno
Bardor, Muriel
author_facet Chuberre, Coralie
Chan, Philippe
Walet-Balieu, Marie-Laure
Thiébert, François
Burel, Carole
Hardouin, Julie
Gügi, Bruno
Bardor, Muriel
author_sort Chuberre, Coralie
collection PubMed
description Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate, and oval. Such pleomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to the comparison of each morphotype issued from one specific strain are scarce and little information is available regarding the physiological significance of this morphogenesis. In this study, we performed a comparative proteomic analysis of the three morphotypes from P. tricornutum. Cultures highly enriched in one dominant morphotype (fusiform, triradiate, or oval) of P. tricornutum Pt3 strain were used. Pairwise comparisons highlighted biological processes, which are up- and down-regulated in the oval (e.g., purine and cellular amino acid metabolism) and triradiate morphotypes (e.g., oxido-reduction and glycolytic processes) compared to the fusiform one used as a reference. Intersection analysis allowed us to identify the specific features of the oval morphotype. Results from this study confirmed previous transcriptomic RNA sequencing observation showing that the oval cells present a distinct metabolism with specific protein enrichment compared to fusiform and triradiate cells. Finally, the analysis of the secretome of each morphotype was also performed.
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spelling pubmed-89777832022-04-05 Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes Chuberre, Coralie Chan, Philippe Walet-Balieu, Marie-Laure Thiébert, François Burel, Carole Hardouin, Julie Gügi, Bruno Bardor, Muriel Front Plant Sci Plant Science Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate, and oval. Such pleomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to the comparison of each morphotype issued from one specific strain are scarce and little information is available regarding the physiological significance of this morphogenesis. In this study, we performed a comparative proteomic analysis of the three morphotypes from P. tricornutum. Cultures highly enriched in one dominant morphotype (fusiform, triradiate, or oval) of P. tricornutum Pt3 strain were used. Pairwise comparisons highlighted biological processes, which are up- and down-regulated in the oval (e.g., purine and cellular amino acid metabolism) and triradiate morphotypes (e.g., oxido-reduction and glycolytic processes) compared to the fusiform one used as a reference. Intersection analysis allowed us to identify the specific features of the oval morphotype. Results from this study confirmed previous transcriptomic RNA sequencing observation showing that the oval cells present a distinct metabolism with specific protein enrichment compared to fusiform and triradiate cells. Finally, the analysis of the secretome of each morphotype was also performed. Frontiers Media S.A. 2022-03-21 /pmc/articles/PMC8977783/ /pubmed/35386671 http://dx.doi.org/10.3389/fpls.2022.673113 Text en Copyright © 2022 Chuberre, Chan, Walet-Balieu, Thiébert, Burel, Hardouin, Gügi and Bardor. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chuberre, Coralie
Chan, Philippe
Walet-Balieu, Marie-Laure
Thiébert, François
Burel, Carole
Hardouin, Julie
Gügi, Bruno
Bardor, Muriel
Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
title Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
title_full Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
title_fullStr Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
title_full_unstemmed Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
title_short Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
title_sort comparative proteomic analysis of the diatom phaeodactylum tricornutum reveals new insights into intra- and extra-cellular protein contents of its oval, fusiform, and triradiate morphotypes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977783/
https://www.ncbi.nlm.nih.gov/pubmed/35386671
http://dx.doi.org/10.3389/fpls.2022.673113
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