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Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold

Angiogenesis, the formation of new capillaries from existing ones, is a fundamental process in regenerative medicine and tissue engineering. While it is known to be affected by circadian rhythms in vivo, its peripheral regulation within the vasculature and the role it performs in regulating the inte...

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Autores principales: Mastrullo, Valeria, van der Veen, Daan R., Gupta, Priyanka, Matos, Rolando S., Johnston, Jonathan D., McVey, John H., Madeddu, Paolo, Velliou, Eirini G., Campagnolo, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977840/
https://www.ncbi.nlm.nih.gov/pubmed/35387328
http://dx.doi.org/10.3389/fphar.2022.867070
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author Mastrullo, Valeria
van der Veen, Daan R.
Gupta, Priyanka
Matos, Rolando S.
Johnston, Jonathan D.
McVey, John H.
Madeddu, Paolo
Velliou, Eirini G.
Campagnolo, Paola
author_facet Mastrullo, Valeria
van der Veen, Daan R.
Gupta, Priyanka
Matos, Rolando S.
Johnston, Jonathan D.
McVey, John H.
Madeddu, Paolo
Velliou, Eirini G.
Campagnolo, Paola
author_sort Mastrullo, Valeria
collection PubMed
description Angiogenesis, the formation of new capillaries from existing ones, is a fundamental process in regenerative medicine and tissue engineering. While it is known to be affected by circadian rhythms in vivo, its peripheral regulation within the vasculature and the role it performs in regulating the interplay between vascular cells have not yet been investigated. Peripheral clocks within the vasculature have been described in the endothelium and in smooth muscle cells. However, to date, scarce evidence has been presented regarding pericytes, a perivascular cell population deeply involved in the regulation of angiogenesis and vessel maturation, as well as endothelial function and homeostasis. More crucially, pericytes are also a promising source of cells for cell therapy and tissue engineering. Here, we established that human primary pericytes express key circadian genes and proteins in a rhythmic fashion upon synchronization. Conversely, we did not detect the same patterns in cultured endothelial cells. In line with these results, pericytes’ viability was disproportionately affected by circadian cycle disruption, as compared to endothelial cells. Interestingly, endothelial cells’ rhythm could be induced following exposure to synchronized pericytes in a contact co-culture. We propose that this mechanism could be linked to the altered release/uptake pattern of lactate, a known mediator of cell-cell interaction which was specifically altered in pericytes by the knockout of the key circadian regulator Bmal1. In an angiogenesis assay, the maturation of vessel-like structures was affected only when both endothelial cells and pericytes did not express Bmal1, indicating a compensation system. In a 3D tissue engineering scaffold, a synchronized clock supported a more structured organization of cells around the scaffold pores, and a maturation of vascular structures. Our results demonstrate that pericytes play a critical role in regulating the circadian rhythms in endothelial cells, and that silencing this system disproportionately affects their pro-angiogenic function. Particularly, in the context of tissue engineering and regenerative medicine, considering the effect of circadian rhythms may be critical for the development of mature vascular structures and to obtain the maximal reparative effect.
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spelling pubmed-89778402022-04-05 Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold Mastrullo, Valeria van der Veen, Daan R. Gupta, Priyanka Matos, Rolando S. Johnston, Jonathan D. McVey, John H. Madeddu, Paolo Velliou, Eirini G. Campagnolo, Paola Front Pharmacol Pharmacology Angiogenesis, the formation of new capillaries from existing ones, is a fundamental process in regenerative medicine and tissue engineering. While it is known to be affected by circadian rhythms in vivo, its peripheral regulation within the vasculature and the role it performs in regulating the interplay between vascular cells have not yet been investigated. Peripheral clocks within the vasculature have been described in the endothelium and in smooth muscle cells. However, to date, scarce evidence has been presented regarding pericytes, a perivascular cell population deeply involved in the regulation of angiogenesis and vessel maturation, as well as endothelial function and homeostasis. More crucially, pericytes are also a promising source of cells for cell therapy and tissue engineering. Here, we established that human primary pericytes express key circadian genes and proteins in a rhythmic fashion upon synchronization. Conversely, we did not detect the same patterns in cultured endothelial cells. In line with these results, pericytes’ viability was disproportionately affected by circadian cycle disruption, as compared to endothelial cells. Interestingly, endothelial cells’ rhythm could be induced following exposure to synchronized pericytes in a contact co-culture. We propose that this mechanism could be linked to the altered release/uptake pattern of lactate, a known mediator of cell-cell interaction which was specifically altered in pericytes by the knockout of the key circadian regulator Bmal1. In an angiogenesis assay, the maturation of vessel-like structures was affected only when both endothelial cells and pericytes did not express Bmal1, indicating a compensation system. In a 3D tissue engineering scaffold, a synchronized clock supported a more structured organization of cells around the scaffold pores, and a maturation of vascular structures. Our results demonstrate that pericytes play a critical role in regulating the circadian rhythms in endothelial cells, and that silencing this system disproportionately affects their pro-angiogenic function. Particularly, in the context of tissue engineering and regenerative medicine, considering the effect of circadian rhythms may be critical for the development of mature vascular structures and to obtain the maximal reparative effect. Frontiers Media S.A. 2022-03-21 /pmc/articles/PMC8977840/ /pubmed/35387328 http://dx.doi.org/10.3389/fphar.2022.867070 Text en Copyright © 2022 Mastrullo, van der Veen, Gupta, Matos, Johnston, McVey, Madeddu, Velliou and Campagnolo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Mastrullo, Valeria
van der Veen, Daan R.
Gupta, Priyanka
Matos, Rolando S.
Johnston, Jonathan D.
McVey, John H.
Madeddu, Paolo
Velliou, Eirini G.
Campagnolo, Paola
Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
title Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
title_full Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
title_fullStr Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
title_full_unstemmed Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
title_short Pericytes’ Circadian Clock Affects Endothelial Cells’ Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
title_sort pericytes’ circadian clock affects endothelial cells’ synchronization and angiogenesis in a 3d tissue engineered scaffold
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977840/
https://www.ncbi.nlm.nih.gov/pubmed/35387328
http://dx.doi.org/10.3389/fphar.2022.867070
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