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Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues
OBJECTIVE: To develop an efficient, clinical-grade, freezing protocol toward experimental clinical cryopreservation of testicular tissues in prepubertal boys suffering from cancer. DESIGN: Experimental cryopreservation of testicular tissue. SETTING: University Medical Center. PATIENT(S): Adult patie...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978083/ https://www.ncbi.nlm.nih.gov/pubmed/35386499 http://dx.doi.org/10.1016/j.xfre.2021.11.001 |
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author | Kabiri, Doron Safrai, Myriam Gropp, Michal Hidas, Guy Mordechai-Daniel, Talya Meir, Karen Revel, Ariel Imbar, Tal Reubinoff, Benjamin |
author_facet | Kabiri, Doron Safrai, Myriam Gropp, Michal Hidas, Guy Mordechai-Daniel, Talya Meir, Karen Revel, Ariel Imbar, Tal Reubinoff, Benjamin |
author_sort | Kabiri, Doron |
collection | PubMed |
description | OBJECTIVE: To develop an efficient, clinical-grade, freezing protocol toward experimental clinical cryopreservation of testicular tissues in prepubertal boys suffering from cancer. DESIGN: Experimental cryopreservation of testicular tissue. SETTING: University Medical Center. PATIENT(S): Adult patients undergoing orchiectomy for various tumors and prepubertal boys scheduled for gonadotoxic treatment. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Histopathological analysis of tissue architecture, structural integrity, and cellular morphology was performed for control and frozen-thawed cryopreserved tissues. The number of seminiferous tubules per testicular section was calculated. The survival of spermatogonial stem cells (SSCs) and Sertoli cells of the control and frozen-thawed cryopreserved tissues was analyzed by immunofluorescence staining. RESULT(S): Uncontrolled Slow Freezing, Controlled slow freezing, and vitrification similarly preserved the integrity of the adult testicular tissues and the survival of SSCs and Sertoli cells. Controlled slow freezing of prepubertal testicular tissues effectively preserved their architecture, the number of tubules, SSCs, and Sertoli cells. In addition, we observed SSC loss after chemotherapy in prepubertal boys, reemphasizing the importance of fertility preservation before gonadotoxic treatment. CONCLUSION(S): Future fertility restoration for male survivors of pediatric cancers depends on the development of an optimal prepubertal testicular tissue cryopreservation method. Our findings demonstrate the effectiveness of controlled slow freezing for cryopreservation of human prepubertal testicular tissues and may contribute to more effective banking of these tissues and potential fertility restoration. CLINICAL TRIAL REGISTRATION NUMBER: NIH research clinical trials number: NCT02529826. |
format | Online Article Text |
id | pubmed-8978083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89780832022-04-05 Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues Kabiri, Doron Safrai, Myriam Gropp, Michal Hidas, Guy Mordechai-Daniel, Talya Meir, Karen Revel, Ariel Imbar, Tal Reubinoff, Benjamin F S Rep Original Article OBJECTIVE: To develop an efficient, clinical-grade, freezing protocol toward experimental clinical cryopreservation of testicular tissues in prepubertal boys suffering from cancer. DESIGN: Experimental cryopreservation of testicular tissue. SETTING: University Medical Center. PATIENT(S): Adult patients undergoing orchiectomy for various tumors and prepubertal boys scheduled for gonadotoxic treatment. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Histopathological analysis of tissue architecture, structural integrity, and cellular morphology was performed for control and frozen-thawed cryopreserved tissues. The number of seminiferous tubules per testicular section was calculated. The survival of spermatogonial stem cells (SSCs) and Sertoli cells of the control and frozen-thawed cryopreserved tissues was analyzed by immunofluorescence staining. RESULT(S): Uncontrolled Slow Freezing, Controlled slow freezing, and vitrification similarly preserved the integrity of the adult testicular tissues and the survival of SSCs and Sertoli cells. Controlled slow freezing of prepubertal testicular tissues effectively preserved their architecture, the number of tubules, SSCs, and Sertoli cells. In addition, we observed SSC loss after chemotherapy in prepubertal boys, reemphasizing the importance of fertility preservation before gonadotoxic treatment. CONCLUSION(S): Future fertility restoration for male survivors of pediatric cancers depends on the development of an optimal prepubertal testicular tissue cryopreservation method. Our findings demonstrate the effectiveness of controlled slow freezing for cryopreservation of human prepubertal testicular tissues and may contribute to more effective banking of these tissues and potential fertility restoration. CLINICAL TRIAL REGISTRATION NUMBER: NIH research clinical trials number: NCT02529826. Elsevier 2021-11-05 /pmc/articles/PMC8978083/ /pubmed/35386499 http://dx.doi.org/10.1016/j.xfre.2021.11.001 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Kabiri, Doron Safrai, Myriam Gropp, Michal Hidas, Guy Mordechai-Daniel, Talya Meir, Karen Revel, Ariel Imbar, Tal Reubinoff, Benjamin Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
title | Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
title_full | Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
title_fullStr | Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
title_full_unstemmed | Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
title_short | Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
title_sort | establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978083/ https://www.ncbi.nlm.nih.gov/pubmed/35386499 http://dx.doi.org/10.1016/j.xfre.2021.11.001 |
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