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Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome
BACKGROUND: Antibodies against thyroid peroxidase (anti-TPO) serve as clinical markers of thyroid autoimmune diseases (TAIDs). By trying to elucidate the causes of heterogeneity in autoantibody levels among patients with different TAIDs it becomes possible to clarify the pathophysiology of GD and HT...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978093/ https://www.ncbi.nlm.nih.gov/pubmed/35386421 http://dx.doi.org/10.1016/j.jcte.2022.100293 |
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author | Espenbetova, Maira Kuzmina, Nina Zubkov, Alexandr Akhmetova, Venera Zamanbekova, Zhanar Krykpaeva, Ainur Zhumanbayeva, Zhanar Amrenova, Kuralay Smailova, Zhanargul Glushkova, Natalya |
author_facet | Espenbetova, Maira Kuzmina, Nina Zubkov, Alexandr Akhmetova, Venera Zamanbekova, Zhanar Krykpaeva, Ainur Zhumanbayeva, Zhanar Amrenova, Kuralay Smailova, Zhanargul Glushkova, Natalya |
author_sort | Espenbetova, Maira |
collection | PubMed |
description | BACKGROUND: Antibodies against thyroid peroxidase (anti-TPO) serve as clinical markers of thyroid autoimmune diseases (TAIDs). By trying to elucidate the causes of heterogeneity in autoantibody levels among patients with different TAIDs it becomes possible to clarify the pathophysiology of GD and HT. OBJECTIVE: To investigate the heterogeneity of epitopes recognized by anti-TPO in patients with Hashimoto’s thyroiditis (HT), Graves’ disease (GD) and overlap-syndrome. METHODS: We carried out a cross-sectional study on 398 patients with GD, HT and overlap syndrome and analyzed the specificity of epitopes and binding constants of TPO with monoclonal antibodies (MAbs). Ten MAbs to TPO were used, of which five were reactive with native TPO and the rest were reactive with denaturated TPO. RESULTS: The autoantibodies in blood serum of HT patients inhibited the binding of MAb63 more significantly than those in serum of GD patients: 59.62 % versus 54.02 %, respectively (p = 0.001). The anti-TPOs in serum of GD patients inhibited the binding of MAb77 more significantly than those in serum of HT patients: 54.36 % versus 51.13 %, respectively (p = 0.047). The binding of MAb45 was more inhibited in serum of patients with anti-TPO concentration over 1000 IU/ml (58.36 %). The blood serum of patients with overlap-syndrome showed less significant inhibition of MAb63 binding than that of patients with no overlap-syndrome: 52.47 % versus 58.81 %, respectively (p = 0.043). CONCLUSION: Mapping the epitopes to TPO with the help of MAbs may improve the differential diagnosis between different thyroid autoimmunities. |
format | Online Article Text |
id | pubmed-8978093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89780932022-04-05 Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome Espenbetova, Maira Kuzmina, Nina Zubkov, Alexandr Akhmetova, Venera Zamanbekova, Zhanar Krykpaeva, Ainur Zhumanbayeva, Zhanar Amrenova, Kuralay Smailova, Zhanargul Glushkova, Natalya J Clin Transl Endocrinol Research Paper BACKGROUND: Antibodies against thyroid peroxidase (anti-TPO) serve as clinical markers of thyroid autoimmune diseases (TAIDs). By trying to elucidate the causes of heterogeneity in autoantibody levels among patients with different TAIDs it becomes possible to clarify the pathophysiology of GD and HT. OBJECTIVE: To investigate the heterogeneity of epitopes recognized by anti-TPO in patients with Hashimoto’s thyroiditis (HT), Graves’ disease (GD) and overlap-syndrome. METHODS: We carried out a cross-sectional study on 398 patients with GD, HT and overlap syndrome and analyzed the specificity of epitopes and binding constants of TPO with monoclonal antibodies (MAbs). Ten MAbs to TPO were used, of which five were reactive with native TPO and the rest were reactive with denaturated TPO. RESULTS: The autoantibodies in blood serum of HT patients inhibited the binding of MAb63 more significantly than those in serum of GD patients: 59.62 % versus 54.02 %, respectively (p = 0.001). The anti-TPOs in serum of GD patients inhibited the binding of MAb77 more significantly than those in serum of HT patients: 54.36 % versus 51.13 %, respectively (p = 0.047). The binding of MAb45 was more inhibited in serum of patients with anti-TPO concentration over 1000 IU/ml (58.36 %). The blood serum of patients with overlap-syndrome showed less significant inhibition of MAb63 binding than that of patients with no overlap-syndrome: 52.47 % versus 58.81 %, respectively (p = 0.043). CONCLUSION: Mapping the epitopes to TPO with the help of MAbs may improve the differential diagnosis between different thyroid autoimmunities. Elsevier 2022-02-04 /pmc/articles/PMC8978093/ /pubmed/35386421 http://dx.doi.org/10.1016/j.jcte.2022.100293 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Espenbetova, Maira Kuzmina, Nina Zubkov, Alexandr Akhmetova, Venera Zamanbekova, Zhanar Krykpaeva, Ainur Zhumanbayeva, Zhanar Amrenova, Kuralay Smailova, Zhanargul Glushkova, Natalya Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome |
title | Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome |
title_full | Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome |
title_fullStr | Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome |
title_full_unstemmed | Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome |
title_short | Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimoto’s thyroiditis and overlap-syndrome |
title_sort | epitopes specificity of antibodies to thyroid peroxidase in patients with graves’ disease, hashimoto’s thyroiditis and overlap-syndrome |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978093/ https://www.ncbi.nlm.nih.gov/pubmed/35386421 http://dx.doi.org/10.1016/j.jcte.2022.100293 |
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