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PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice

Plastics are used commonly in the world because of their convenience and cost effectiveness. Microplastics, an environmental threat and human health risk, are widely detected in food and consequently ingested. However, degraded plastics are found everywhere, creating an environmental threat and huma...

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Autores principales: Im, Changkeun, Kim, Hyeongi, Zaheer, Javeria, Kim, Jung Young, Lee, Yong-Jin, Kang, Choong Mo, Kim, Jin Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Nuclear Medicine 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978192/
https://www.ncbi.nlm.nih.gov/pubmed/34215675
http://dx.doi.org/10.2967/jnumed.120.256982
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author Im, Changkeun
Kim, Hyeongi
Zaheer, Javeria
Kim, Jung Young
Lee, Yong-Jin
Kang, Choong Mo
Kim, Jin Su
author_facet Im, Changkeun
Kim, Hyeongi
Zaheer, Javeria
Kim, Jung Young
Lee, Yong-Jin
Kang, Choong Mo
Kim, Jin Su
author_sort Im, Changkeun
collection PubMed
description Plastics are used commonly in the world because of their convenience and cost effectiveness. Microplastics, an environmental threat and human health risk, are widely detected in food and consequently ingested. However, degraded plastics are found everywhere, creating an environmental threat and human health risk. Therefore, real-time monitoring of orally administered microplastics to trace them in the body is tremendously important. Methods: In this study, to visualize their absorption path, we labeled polystyrene with [(64)Cu]Cu-DOTA. We prepared radiolabeled polystyrene with (64)Cu. Afterward, [(64)Cu]Cu-DOTA-polystyrene was orally administered to mice, and we evaluated its transit and absorption using PET imaging. The absorption path and distribution of [(64)Cu]Cu-DOTA-polystyrene were determined using PET over 48 h. Ex vivo tissue radio–thin-layer chromatography (TLC) was used to demonstrate the existence of [(64)Cu]Cu-DOTA-polystyrene in tissue. Results: PET images demonstrated that [(64)Cu]Cu-DOTA-polystyrene began to transit to the intestine within 1 h. Accumulation of [(64)Cu]Cu-DOTA-polystyrene in the liver was also observed. The biodistribution of [(64)Cu]Cu-DOTA-polystyrene confirmed the distribution of [(64)Cu]Cu-DOTA-polystyrene observed on the PET images. Ex vivo radio-TLC demonstrated that the detected γ-rays originated from [(64)Cu]Cu-DOTA-polystyrene. Conclusion: This study provided PET evidence of the existence and accumulation of microplastics in tissue and cross-confirmed the PET findings by ex vivo radio-TLC. This information may be used as the basis for future studies on the toxicity of microplastics.
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spelling pubmed-89781922022-04-15 PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice Im, Changkeun Kim, Hyeongi Zaheer, Javeria Kim, Jung Young Lee, Yong-Jin Kang, Choong Mo Kim, Jin Su J Nucl Med Focus on Molecular Imaging Plastics are used commonly in the world because of their convenience and cost effectiveness. Microplastics, an environmental threat and human health risk, are widely detected in food and consequently ingested. However, degraded plastics are found everywhere, creating an environmental threat and human health risk. Therefore, real-time monitoring of orally administered microplastics to trace them in the body is tremendously important. Methods: In this study, to visualize their absorption path, we labeled polystyrene with [(64)Cu]Cu-DOTA. We prepared radiolabeled polystyrene with (64)Cu. Afterward, [(64)Cu]Cu-DOTA-polystyrene was orally administered to mice, and we evaluated its transit and absorption using PET imaging. The absorption path and distribution of [(64)Cu]Cu-DOTA-polystyrene were determined using PET over 48 h. Ex vivo tissue radio–thin-layer chromatography (TLC) was used to demonstrate the existence of [(64)Cu]Cu-DOTA-polystyrene in tissue. Results: PET images demonstrated that [(64)Cu]Cu-DOTA-polystyrene began to transit to the intestine within 1 h. Accumulation of [(64)Cu]Cu-DOTA-polystyrene in the liver was also observed. The biodistribution of [(64)Cu]Cu-DOTA-polystyrene confirmed the distribution of [(64)Cu]Cu-DOTA-polystyrene observed on the PET images. Ex vivo radio-TLC demonstrated that the detected γ-rays originated from [(64)Cu]Cu-DOTA-polystyrene. Conclusion: This study provided PET evidence of the existence and accumulation of microplastics in tissue and cross-confirmed the PET findings by ex vivo radio-TLC. This information may be used as the basis for future studies on the toxicity of microplastics. Society of Nuclear Medicine 2022-03 /pmc/articles/PMC8978192/ /pubmed/34215675 http://dx.doi.org/10.2967/jnumed.120.256982 Text en © 2022 by the Society of Nuclear Medicine and Molecular Imaging. https://creativecommons.org/licenses/by/4.0/Immediate Open Access: Creative Commons Attribution 4.0 International License (CC BY) allows users to share and adapt with attribution, excluding materials credited to previous publications. License: https://creativecommons.org/licenses/by/4.0/. Details: http://jnm.snmjournals.org/site/misc/permission.xhtml.
spellingShingle Focus on Molecular Imaging
Im, Changkeun
Kim, Hyeongi
Zaheer, Javeria
Kim, Jung Young
Lee, Yong-Jin
Kang, Choong Mo
Kim, Jin Su
PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice
title PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice
title_full PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice
title_fullStr PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice
title_full_unstemmed PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice
title_short PET Tracing of Biodistribution for Orally Administered (64)Cu-Labeled Polystyrene in Mice
title_sort pet tracing of biodistribution for orally administered (64)cu-labeled polystyrene in mice
topic Focus on Molecular Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978192/
https://www.ncbi.nlm.nih.gov/pubmed/34215675
http://dx.doi.org/10.2967/jnumed.120.256982
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