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Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p

BACKGROUND: The characteristics of muscle fibers determine the growth and meat quality of poultry. In this study, we performed a weighted gene co-expression network analysis (WGCNA) on the muscle fiber characteristics and transcriptome profile of the breast muscle tissue of Gushi chicken at 6, 14, 2...

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Autores principales: Li, Yuanfang, Yuan, Pengtao, Fan, Shengxin, Zhai, Bin, Jin, Wenjiao, Li, Donghua, Li, Hong, Sun, Guirong, Han, Ruili, Liu, Xiaojun, Tian, Yadong, Li, Guoxi, Kang, Xiangtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978428/
https://www.ncbi.nlm.nih.gov/pubmed/35379193
http://dx.doi.org/10.1186/s12864-022-08522-8
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author Li, Yuanfang
Yuan, Pengtao
Fan, Shengxin
Zhai, Bin
Jin, Wenjiao
Li, Donghua
Li, Hong
Sun, Guirong
Han, Ruili
Liu, Xiaojun
Tian, Yadong
Li, Guoxi
Kang, Xiangtao
author_facet Li, Yuanfang
Yuan, Pengtao
Fan, Shengxin
Zhai, Bin
Jin, Wenjiao
Li, Donghua
Li, Hong
Sun, Guirong
Han, Ruili
Liu, Xiaojun
Tian, Yadong
Li, Guoxi
Kang, Xiangtao
author_sort Li, Yuanfang
collection PubMed
description BACKGROUND: The characteristics of muscle fibers determine the growth and meat quality of poultry. In this study, we performed a weighted gene co-expression network analysis (WGCNA) on the muscle fiber characteristics and transcriptome profile of the breast muscle tissue of Gushi chicken at 6, 14, 22, and 30 weeks. RESULTS: A total of 27 coexpressed biological functional modules were identified, of which the midnight blue module had the strongest correlation with muscle fiber and diameter. In addition, 7 hub genes were found from the midnight blue module, including LC8 dynein light chain 2 (DYNLL2). Combined with miRNA transcriptome data, miR-148a-3p was found to be a potential target miRNA of DYNLL2. Experiments on chicken primary myoblasts (CPMs) demonstrated that miR-148a-3p promotes the expression of myosin heavy chain (MYHC) protein by targeting DYNLL2, proving that it can promote differentiation of myoblasts. CONCLUSIONS: This study proved that the hub gene DYNLL2 and its target miR-148-3p are important regulators in chicken myogenesis. These results provide novel insights for understanding the molecular regulation mechanisms related to the development of chicken breast muscle. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08522-8.
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spelling pubmed-89784282022-04-05 Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p Li, Yuanfang Yuan, Pengtao Fan, Shengxin Zhai, Bin Jin, Wenjiao Li, Donghua Li, Hong Sun, Guirong Han, Ruili Liu, Xiaojun Tian, Yadong Li, Guoxi Kang, Xiangtao BMC Genomics Research BACKGROUND: The characteristics of muscle fibers determine the growth and meat quality of poultry. In this study, we performed a weighted gene co-expression network analysis (WGCNA) on the muscle fiber characteristics and transcriptome profile of the breast muscle tissue of Gushi chicken at 6, 14, 22, and 30 weeks. RESULTS: A total of 27 coexpressed biological functional modules were identified, of which the midnight blue module had the strongest correlation with muscle fiber and diameter. In addition, 7 hub genes were found from the midnight blue module, including LC8 dynein light chain 2 (DYNLL2). Combined with miRNA transcriptome data, miR-148a-3p was found to be a potential target miRNA of DYNLL2. Experiments on chicken primary myoblasts (CPMs) demonstrated that miR-148a-3p promotes the expression of myosin heavy chain (MYHC) protein by targeting DYNLL2, proving that it can promote differentiation of myoblasts. CONCLUSIONS: This study proved that the hub gene DYNLL2 and its target miR-148-3p are important regulators in chicken myogenesis. These results provide novel insights for understanding the molecular regulation mechanisms related to the development of chicken breast muscle. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08522-8. BioMed Central 2022-04-04 /pmc/articles/PMC8978428/ /pubmed/35379193 http://dx.doi.org/10.1186/s12864-022-08522-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Yuanfang
Yuan, Pengtao
Fan, Shengxin
Zhai, Bin
Jin, Wenjiao
Li, Donghua
Li, Hong
Sun, Guirong
Han, Ruili
Liu, Xiaojun
Tian, Yadong
Li, Guoxi
Kang, Xiangtao
Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p
title Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p
title_full Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p
title_fullStr Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p
title_full_unstemmed Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p
title_short Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p
title_sort weighted gene co-expression network indicates that the dynll2 is an important regulator of chicken breast muscle development and is regulated by mir-148a-3p
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978428/
https://www.ncbi.nlm.nih.gov/pubmed/35379193
http://dx.doi.org/10.1186/s12864-022-08522-8
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