Cargando…

Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli

Nanostructures exhibit a bactericidal effect owing to physical interaction with the bacterial cell envelope. Here, we aimed to identify the mechanism underlying the bactericidal effect of nanostructures based on bacterial autolysis, in contrast to previous reports focusing on structural characterist...

Descripción completa

Detalles Bibliográficos
Autores principales: Mimura, Soma, Shimizu, Tomohiro, Shingubara, Shoso, Iwaki, Hiroaki, Ito, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978875/
https://www.ncbi.nlm.nih.gov/pubmed/35425160
http://dx.doi.org/10.1039/d1ra07623j
_version_ 1784681050153156608
author Mimura, Soma
Shimizu, Tomohiro
Shingubara, Shoso
Iwaki, Hiroaki
Ito, Takeshi
author_facet Mimura, Soma
Shimizu, Tomohiro
Shingubara, Shoso
Iwaki, Hiroaki
Ito, Takeshi
author_sort Mimura, Soma
collection PubMed
description Nanostructures exhibit a bactericidal effect owing to physical interaction with the bacterial cell envelope. Here, we aimed to identify the mechanism underlying the bactericidal effect of nanostructures based on bacterial autolysis, in contrast to previous reports focusing on structural characteristics. The time profiles of active cell ratios of the Escherichia coli strains (WT, ΔmltA, ΔmltB, Δslt70), incubation time of the wild-type (WT) strains, and autolysis inhibition of WT strains were evaluated with respect to the bactericidal effect of the applied nanostructures. Addition of Mg(2+), an autolysis inhibitor, was not found to cause significant cell damage. The incubation phase was significantly associated with envelope damage. The lytic transglycosylase-lacking strain of Slt70 (Δslt70) also showed only minimal envelope damage. Our results indicate that nanostructures may act by triggering bacterial autolysis.
format Online
Article
Text
id pubmed-8978875
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-89788752022-04-13 Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli Mimura, Soma Shimizu, Tomohiro Shingubara, Shoso Iwaki, Hiroaki Ito, Takeshi RSC Adv Chemistry Nanostructures exhibit a bactericidal effect owing to physical interaction with the bacterial cell envelope. Here, we aimed to identify the mechanism underlying the bactericidal effect of nanostructures based on bacterial autolysis, in contrast to previous reports focusing on structural characteristics. The time profiles of active cell ratios of the Escherichia coli strains (WT, ΔmltA, ΔmltB, Δslt70), incubation time of the wild-type (WT) strains, and autolysis inhibition of WT strains were evaluated with respect to the bactericidal effect of the applied nanostructures. Addition of Mg(2+), an autolysis inhibitor, was not found to cause significant cell damage. The incubation phase was significantly associated with envelope damage. The lytic transglycosylase-lacking strain of Slt70 (Δslt70) also showed only minimal envelope damage. Our results indicate that nanostructures may act by triggering bacterial autolysis. The Royal Society of Chemistry 2022-01-10 /pmc/articles/PMC8978875/ /pubmed/35425160 http://dx.doi.org/10.1039/d1ra07623j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mimura, Soma
Shimizu, Tomohiro
Shingubara, Shoso
Iwaki, Hiroaki
Ito, Takeshi
Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli
title Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli
title_full Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli
title_fullStr Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli
title_full_unstemmed Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli
title_short Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli
title_sort bactericidal effect of nanostructures via lytic transglycosylases of escherichia coli
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978875/
https://www.ncbi.nlm.nih.gov/pubmed/35425160
http://dx.doi.org/10.1039/d1ra07623j
work_keys_str_mv AT mimurasoma bactericidaleffectofnanostructuresvialytictransglycosylasesofescherichiacoli
AT shimizutomohiro bactericidaleffectofnanostructuresvialytictransglycosylasesofescherichiacoli
AT shingubarashoso bactericidaleffectofnanostructuresvialytictransglycosylasesofescherichiacoli
AT iwakihiroaki bactericidaleffectofnanostructuresvialytictransglycosylasesofescherichiacoli
AT itotakeshi bactericidaleffectofnanostructuresvialytictransglycosylasesofescherichiacoli