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Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells

PURPOSE: Nanoparticles are resources of advanced nanotechnology being present in several products. Titanium dioxide nanoparticles are among the five most widely used NP currently expanding their benefits from the oil industry to the areas of diagnostic medicine due to their properties and small size...

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Autores principales: Brassolatti, Patricia, de Almeida Rodolpho, Joice Margareth, Franco de Godoy, Krissia, de Castro, Cynthia Aparecida, Flores Luna, Genoveva Lourdes, Dias de Lima Fragelli, Bruna, Pedrino, Matheus, Assis, Marcelo, Nani Leite, Marcel, Cancino-Bernardi, Juliana, Speglich, Carlos, Frade, Marco Andrey, de Freitas Anibal, Fernanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978907/
https://www.ncbi.nlm.nih.gov/pubmed/35388270
http://dx.doi.org/10.2147/IJN.S325767
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author Brassolatti, Patricia
de Almeida Rodolpho, Joice Margareth
Franco de Godoy, Krissia
de Castro, Cynthia Aparecida
Flores Luna, Genoveva Lourdes
Dias de Lima Fragelli, Bruna
Pedrino, Matheus
Assis, Marcelo
Nani Leite, Marcel
Cancino-Bernardi, Juliana
Speglich, Carlos
Frade, Marco Andrey
de Freitas Anibal, Fernanda
author_facet Brassolatti, Patricia
de Almeida Rodolpho, Joice Margareth
Franco de Godoy, Krissia
de Castro, Cynthia Aparecida
Flores Luna, Genoveva Lourdes
Dias de Lima Fragelli, Bruna
Pedrino, Matheus
Assis, Marcelo
Nani Leite, Marcel
Cancino-Bernardi, Juliana
Speglich, Carlos
Frade, Marco Andrey
de Freitas Anibal, Fernanda
author_sort Brassolatti, Patricia
collection PubMed
description PURPOSE: Nanoparticles are resources of advanced nanotechnology being present in several products. Titanium dioxide nanoparticles are among the five most widely used NP currently expanding their benefits from the oil industry to the areas of diagnostic medicine due to their properties and small size. However, its impact on human health is still controversial in the literature. We aimed to evaluate the cytotoxicity of a new titanium NP functionalized with sodium carboxylic ligand (COOH(−)Na(+)) in human keratinocytes (HaCaT) and human fibroblasts (HDFn). METHODS: The physical-chemical characterization was performed by the transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential techniques, respectively. MTT and LDH assays were used to assess cytotoxicity and cell membrane damage respectively, ELISA to identify the inflammatory profile and, reactive oxygen species assay and cytometry to detect reactive oxygen species and their relationship with apoptosis/necrosis mechanisms. RESULTS: The results demonstrated a decrease in cell viability at the highest concentrations tested for both cell lines, but no change in LDH release was detected for the HaCaT. The cell membrane damage was found only at 100.0 µg/mL for the HDFn. It was demonstrated that cytotoxicity in the highest concentrations evaluated for both cell lines for the 72 h period. The HDFn showed damage to the cell membrane at a concentration of 100 µg/mL followed by a significant increase in reactive oxygen species production. No inflammatory profile was detected. The HaCaT showed apoptosis when exposed to the highest concentration evaluated and HDFn showed both apoptosis and necrosis for the same concentration. CONCLUSION: Thus, it is possible to conclude that the cytotoxicity mechanism differs according to the cell type evaluated, with HDFn being the most sensitive line in this case, and this mechanism can be defined in a dose and time dependent manner, since the highest concentrations also triggered death cell.
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spelling pubmed-89789072022-04-05 Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells Brassolatti, Patricia de Almeida Rodolpho, Joice Margareth Franco de Godoy, Krissia de Castro, Cynthia Aparecida Flores Luna, Genoveva Lourdes Dias de Lima Fragelli, Bruna Pedrino, Matheus Assis, Marcelo Nani Leite, Marcel Cancino-Bernardi, Juliana Speglich, Carlos Frade, Marco Andrey de Freitas Anibal, Fernanda Int J Nanomedicine Original Research PURPOSE: Nanoparticles are resources of advanced nanotechnology being present in several products. Titanium dioxide nanoparticles are among the five most widely used NP currently expanding their benefits from the oil industry to the areas of diagnostic medicine due to their properties and small size. However, its impact on human health is still controversial in the literature. We aimed to evaluate the cytotoxicity of a new titanium NP functionalized with sodium carboxylic ligand (COOH(−)Na(+)) in human keratinocytes (HaCaT) and human fibroblasts (HDFn). METHODS: The physical-chemical characterization was performed by the transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential techniques, respectively. MTT and LDH assays were used to assess cytotoxicity and cell membrane damage respectively, ELISA to identify the inflammatory profile and, reactive oxygen species assay and cytometry to detect reactive oxygen species and their relationship with apoptosis/necrosis mechanisms. RESULTS: The results demonstrated a decrease in cell viability at the highest concentrations tested for both cell lines, but no change in LDH release was detected for the HaCaT. The cell membrane damage was found only at 100.0 µg/mL for the HDFn. It was demonstrated that cytotoxicity in the highest concentrations evaluated for both cell lines for the 72 h period. The HDFn showed damage to the cell membrane at a concentration of 100 µg/mL followed by a significant increase in reactive oxygen species production. No inflammatory profile was detected. The HaCaT showed apoptosis when exposed to the highest concentration evaluated and HDFn showed both apoptosis and necrosis for the same concentration. CONCLUSION: Thus, it is possible to conclude that the cytotoxicity mechanism differs according to the cell type evaluated, with HDFn being the most sensitive line in this case, and this mechanism can be defined in a dose and time dependent manner, since the highest concentrations also triggered death cell. Dove 2022-03-30 /pmc/articles/PMC8978907/ /pubmed/35388270 http://dx.doi.org/10.2147/IJN.S325767 Text en © 2022 Brassolatti et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Brassolatti, Patricia
de Almeida Rodolpho, Joice Margareth
Franco de Godoy, Krissia
de Castro, Cynthia Aparecida
Flores Luna, Genoveva Lourdes
Dias de Lima Fragelli, Bruna
Pedrino, Matheus
Assis, Marcelo
Nani Leite, Marcel
Cancino-Bernardi, Juliana
Speglich, Carlos
Frade, Marco Andrey
de Freitas Anibal, Fernanda
Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells
title Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells
title_full Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells
title_fullStr Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells
title_full_unstemmed Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells
title_short Functionalized Titanium Nanoparticles Induce Oxidative Stress and Cell Death in Human Skin Cells
title_sort functionalized titanium nanoparticles induce oxidative stress and cell death in human skin cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978907/
https://www.ncbi.nlm.nih.gov/pubmed/35388270
http://dx.doi.org/10.2147/IJN.S325767
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