Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially dis...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yang, DiStasio, Marcello, Su, Graham, Asashima, Hiromitsu, Enninful, Archibald, Qin, Xiaoyu, Deng, Yanxiang, Bordignon, Pino, Cassano, Marco, Tomayko, Mary, Xu, Mina, Halene, Stephanie, Craft, Joseph E., Hafler, David, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978952/
https://www.ncbi.nlm.nih.gov/pubmed/35378748
http://dx.doi.org/10.21203/rs.3.rs-1499315/v1
Descripción
Sumario:We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.

Ejemplares similares