Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially dis...

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Autores principales: Liu, Yang, DiStasio, Marcello, Su, Graham, Asashima, Hiromitsu, Enninful, Archibald, Qin, Xiaoyu, Deng, Yanxiang, Bordignon, Pino, Cassano, Marco, Tomayko, Mary, Xu, Mina, Halene, Stephanie, Craft, Joseph E., Hafler, David, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978952/
https://www.ncbi.nlm.nih.gov/pubmed/35378748
http://dx.doi.org/10.21203/rs.3.rs-1499315/v1
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author Liu, Yang
DiStasio, Marcello
Su, Graham
Asashima, Hiromitsu
Enninful, Archibald
Qin, Xiaoyu
Deng, Yanxiang
Bordignon, Pino
Cassano, Marco
Tomayko, Mary
Xu, Mina
Halene, Stephanie
Craft, Joseph E.
Hafler, David
Fan, Rong
author_facet Liu, Yang
DiStasio, Marcello
Su, Graham
Asashima, Hiromitsu
Enninful, Archibald
Qin, Xiaoyu
Deng, Yanxiang
Bordignon, Pino
Cassano, Marco
Tomayko, Mary
Xu, Mina
Halene, Stephanie
Craft, Joseph E.
Hafler, David
Fan, Rong
author_sort Liu, Yang
collection PubMed
description We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.
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spelling pubmed-89789522022-04-05 Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping Liu, Yang DiStasio, Marcello Su, Graham Asashima, Hiromitsu Enninful, Archibald Qin, Xiaoyu Deng, Yanxiang Bordignon, Pino Cassano, Marco Tomayko, Mary Xu, Mina Halene, Stephanie Craft, Joseph E. Hafler, David Fan, Rong Res Sq Article We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research. American Journal Experts 2022-03-30 /pmc/articles/PMC8978952/ /pubmed/35378748 http://dx.doi.org/10.21203/rs.3.rs-1499315/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Liu, Yang
DiStasio, Marcello
Su, Graham
Asashima, Hiromitsu
Enninful, Archibald
Qin, Xiaoyu
Deng, Yanxiang
Bordignon, Pino
Cassano, Marco
Tomayko, Mary
Xu, Mina
Halene, Stephanie
Craft, Joseph E.
Hafler, David
Fan, Rong
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
title Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
title_full Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
title_fullStr Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
title_full_unstemmed Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
title_short Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
title_sort spatial-cite-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978952/
https://www.ncbi.nlm.nih.gov/pubmed/35378748
http://dx.doi.org/10.21203/rs.3.rs-1499315/v1
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